摘要
目的:建立五香血藤药材的质量测定,为五香血藤的质量标准研究和修订提供借鉴基础。方法:测定药材水分、总灰分和酸不溶性灰分;采用高效液相色谱法测定药材中五味子乙素的含量:色谱柱为Eclipse XDB-C18,流动相为乙腈-水(80∶20,V/V),流速为1.0 m L/min,检测波长为235 nm,柱温为30℃,进样量为20μL。结果:药材水分为10.74~14.49%,总灰分为2.12~4.30%,酸不溶性灰分为0.18~3.16%。五味子乙素检测进样量线性范围为2.10~12.62μg(r=0.9999);精密度、稳定性、重复性试验的RSD≤3%;五香血藤素加样回收率为102.27%(RSD=2.6%)。结论:该研究所建标准可用于五香血藤药材的质量控制。
In order to provide reference for the research and revision of the quality standard of Schisandra rubriflora vine,we developed a method to determine the quality of Schisandra rubriflora vine. We determined the moisture content,total ash content,and acid-insoluble ash content of the medicinal materials. The content of schisandrin B was determined by HPLC with parameters as follows： Eclipse XDB-C18 chromatographic column,acetonitrile-water（ 80∶ 20,V/V） as mobile phase,flow rate of 1. 0 m L/min,detection wavelength of 235 nm,column temperature of 30 ℃,20 μL injection volume. The results showed that the moisture content of the medicinal materials was10. 74% to 14. 49%,total ash content was 2. 12% to 4. 30%,and acid-insoluble ash content was 0. 18% to3. 16%. The linear range of schisandrin B was 2. 10 μg to 12. 62 μg（ r = 0. 9999）; the RSD of precision,stability,and repeatability tests was ≤ 3 %; the recovery rate of schisandrin B was 102. 27%（ RSD = 2. 6%）. The established method could be used for the quality control of Schisandra rubriflora vine.
作者
刘慧
徐剑
LIU Hui;XU Jian(School of Pharmacy,Guiyang University of Chinese Medicine,Guiyang 550025,China)
出处
《贵州科学》
2018年第4期20-23,共4页
Guizhou Science
基金
科技部国家工程技术研究中心组建项目
国家苗药工程技术研究中心(2014FU125Q09)
贵州省普通高等学校中药民族药制剂重点实验室--(黔教合KY字[2016]005)
贵州省优秀青年科技人才项目:基于谱效关系在中药提取工艺中的评价体系建立(黔科合人字[2015]23号)
关键词
五香血藤
高效液相色谱法
质量标准
五味子乙素
Schisandra rubriflora vine
high performance liquid chromatography
quality standard
schisandrin B
