摘要
目的探讨慢性丙型肝炎患者外周血CD3^+CD(16+56)^+自然杀伤细胞(NK细胞)的变化与肝纤维化指标的相关性。方法对65例慢性丙型肝炎组及30例健康对照组采用流式细胞术(FCM)检测外周血NK细胞百分比和绝对值,并采用化学发光法检测血清中肝纤维化四项指标:透明质酸(HA),Ⅲ型前胶原肽(PCⅢ),Ⅳ型胶原(Ⅳ-C)和层黏连蛋白(LN)的水平。同时对患者NK细胞的水平与四项肝纤维化指标含量进行相关性分析。结果慢性丙型肝炎患者NK细胞的总数和百分比低于健康对照组,差异有统计学意义(t=6.204,2.473,均P<0.05);肝纤维化四项指标中HA和Ⅳ-C含量高于健康对照组,差异有统计学意义(t=3.899,4.654,均P<0.05),PCⅢ和LN与健康对照组比较含量无明显变化,差异无统计学意义(t=0.223,0.175,均P>0.05)。同时通过相关性分析得出患者HA和Ⅳ-C含量的变化与NK细胞的水平呈现负相关(r=0.939,P<0.05;r=0.841,P<0.05),PCⅢ和LN含量的变化与NK细胞的水平无相关性(r=-0.062,P>0.05;r=0.051,P>0.05)。结论丙型肝炎病毒感染阶段,NK细胞数量下降与机体清除HCV病毒的能力下降有关;同时NK细胞数量下降使得患者肝脏更易形成纤维化。
Objective To analyse the correlation between the changes of natural killer cell count and the indexes of liver fibrosis in patients with chronic hepatitis C.Methods The total number,the percentage and absolute value of NK cells in peripheral blood of 65 patients with chronic hepatitis C,and 30 healthy control group were detected by flow cytometry.Four indexes of liver fibrosis in serum were detected by chemiluminescence：hyaluronic acid（HA）,type Ⅲ procollagen peptide（PC Ⅲ）,type Ⅳ collagen（Ⅳ-C）and laminin（LN）.At the same time,the correlation between the level of NK cells and the four indexes of liver fibrosis was also studied.Results The total number and percentage of NK cells in patients with chronic hepatitis C were lower than those in healthy controls（t=6.204,2.473,all P〈0.05）.The content of HA and Ⅳ-C was higher than that in the healthy control group,the difference was statistically significant（t=3.899,4.654,all P〈0.05）.There was no significant difference in the content of PC and LN with the healthy control group,and there was no statistical difference（t=0.223,0.175,all P〉0.05）.At the same time,it was found that there was a negative correlation between the changes of HA and Ⅳ-C and the level of NK cells（r=0.939,P〈0.05;r=0.841,P〈0.05）.There was no correlation between the changes of HA and LN indexes and the level of NK cells（r=-0.062,P〉0.05;r=0.051,P〉0.05）.Conclusion In hepatitis C virus infection phase,the decline in the number of NK cells was related to the decline in the ability of the body to clear the HCV virus,NK decreased cell numbers,making it easier for patients to form fibrosis.
作者
吴志美
袁建芬
徐建
许亮亮
WU Zhi-mei;YUAN Jian-fen;XU Jian;XU Liang-liang(Department of Clinical Laboratory,Traditional Chinese Medicine Hospital of Jiangsu Nantong,Jiangsu Nantong 226001,China)
出处
《现代检验医学杂志》
CAS
2018年第4期95-97,共3页
Journal of Modern Laboratory Medicine
