摘要
目的分析研究一名献血者ABO血型形成的分子生物学机制。方法用血清学方法检测ABO和H抗原,应用聚合酶链式反应(polymerase chain reation,PCR)检测其基因型,再对ABO基因第6,7外显子进行直接测序。结果该献血员血清学检测为AB亚型,PCR结果为A2/Bw12,直接测序结果为A205/Bw12。结论该献血员血型是一例比较罕见的AB双侧亚型。为保障输血安全,在该献血员不含抗体的情况下,其血液可以输注给配血相合的AB型患者。
Objective To explore the molecular mechanism of a blood donor＇s ABO blood group.Methods The serological test was applied to test the ABO and H antigens of the blood donor.The polymerase chain reation（PCR）was applied to exam the ABO genotype,and the direct sequencing was applied to test the exon6 and exon7 sequence of the blood donor＇s ABO gene.Results The results of the serological test indicated that the donor＇s ABO blood was AB subtype.The A2/Bw12 genotype was acquired with PCR and the A205/Bw12 genotpye was acquired with the direct sequencing.Conclusion The donor＇s blood type is a rare AB subtype with both A and B are subtypes.
作者
李双玉
董磊
解金辉
赵瑛
LI Shuang-yu;DONG Lei;XIE Jin-hui;ZHAO Ying(Tianjin Blood Center,Tianjin 300110,China)
出处
《现代检验医学杂志》
CAS
2018年第4期70-72,共3页
Journal of Modern Laboratory Medicine
