摘要
目的观察不同储存温度和时间对单采新鲜冰冻血浆速冻前凝血因子Ⅷ活性的变化情况,以采用合适的流程来保障单采新鲜冰冻血浆的质量。方法将即时采集的单采新鲜血浆立即留取20 m L,分装在9支试管里,按照0 h立即速冻、4℃4 h、4℃6 h、4℃8 h、4℃12 h、4℃24 h、室温（23℃±2℃）4 h、室温6 h、室温8 h、室温12 h、室温24 h分组进行低温速冻。速冻标本37℃水浴融化后立即检测凝血因子Ⅷ活性（FⅧ∶C）。结果单采新鲜血浆内的凝血因子Ⅷ活性在冰冻制备前随储存时间的延长而降低;在4℃或室温条件下储存4 h或6 h再制备与0 h制备相比,其中的凝血因子Ⅷ活性无统计学差异（P〉0.05）;4℃或室温放置8 h后再制备与0 h制备比较,凝血因子Ⅷ活性的差异有统计学意义（P〈0.05或P〈0.01）。在4℃与室温条件下储存相同时间,4℃存放凝血因子Ⅷ活性的下降速率低于室温条件;4 h、6 h、8 h、12 h内制备,凝血因子Ⅷ活性的下降速率无显著性差异（P〉0.05）;储存24 h后制备,在室温条件下的凝血因子Ⅷ活性下降速率明显高于4℃条件下（P〈0.01）。结论单采新鲜冰冻血浆储存于4℃或室温下6 h内速冻制备,对其凝血因子Ⅷ活性的影响不大。随着储存时间的延长,活性损失呈递增趋势。因此,储存于4℃或室温下的单采新鲜冰冻血浆最好在6 h内速冻制备,以免影响血液质量。
Objective The influence of storage temperature and duration on coagulation the factor Ⅷ activity in fresh plasma was observed in order to propose appropriate frozen procedure to acquire quality fresh frozen plasma. Methods After apheresis,the fresh plasma was immediately divided into 9 aliquots and added into 9 test tubes（ 20 m L per tube） and stored in the following conditions： room temperature（ RT）/0 h,4℃ 4 h,4℃ 6 h,4℃ 8 h,4℃ 12 h,4℃ 24 h,RT 4 h,RT 6 h,RT 8 h,RT 12 h,and RT 24 h. The samples were processed to detect FⅧ： C immediately after melting in a 37℃ water bath. Results The coagulant factor VIII activity in fresh plasma decreased with the prolongation of storage time. There was no statistically significant difference in coagulant factor VIII activity between the 0 h storage group and the 4 h or 6 h storage at 4℃/RT（ P〈0. 05）. However,comparing to the value at 0 h,coagulant factor VIII activity decreased significantly when fresh plasma was stored for 8 h,12 h and 24 h at 4℃/RT（ P〈0. 05 or 0. 01）. The decline rates of coagulant factor VIII activity was lower in the 4℃ stored groups than the RT groups given the same storage period. However,no significant difference（ P〈0. 05） between the 4℃ and the RT groups were found except for the 24 h storage group（ P〈0. 01）. Conclusion The coagulant factor VIII activity was not affected dramatically if fresh frozen plasma was prepared within 4 h. With the prolonged storage time,the activity showed a decreasing trend. Therefore,we recommend the fresh plasma to be stored at 4 ℃/RT within 6 h to maintain quality blood components.
作者
王静
李洪波
王园园
WANG Jing;LI Hongbo;WANG Yuanyuan(Binzhou Central Blood Station,Binzhou,256200,China)
出处
《中国输血杂志》
CAS
2018年第6期668-670,共3页
Chinese Journal of Blood Transfusion
