摘要
香稻是由于水稻种水稻甜菜碱醛脱氢酶(BADH2)基因的功能缺失突变引起的。根据香味等位基因badh2的功能性突变位点设计的功能性分子标记,为快速鉴定具有香味的水稻种质资源提供了可能。本研究利用香味等位基因badh2-E2和badh2-E7的功能性分子标记InDel-E2和InDel-E7鉴定了33个杂交粳稻不育系和35个杂交粳稻恢复系材料的基因型,获得了12个含有badh2-E2等位基因不育系和4个含有badh2-E7等位基因恢复系材料。在此基础上,分别选择了3个不育系和3个恢复系,配制了5个全香型的杂交粳稻,为香味杂交粳稻的推广应用提供了理论依据。
Fragrant rice is caused by the functional loss and mutation of the Betaine aldehyde aehydrogenase（BADH2） gene.Functional molecular markers designed according to the functional mutation loci of the aroma allele badh2 make it possible for rapid identification the fragrant rice germplasm.In this study,33 male sterile lines and 35 restorer lines of japonica hybrid rice were genotyped by using functional markers InDel-E2 and InDel-E7,which were designed respectively for the fragrant badh2 alleles badh2-E2 and badh2-E7,12 sterile lines containing badh2-E2 allele and 4 restorer lines containing badh2-E7 allele were identified,respectively.On the basis of genotyping result,3 fragrant sterile lines and 3 fragrant restorer lines were selected to develop 5 fragrant japonica hybrid recombination.These results might provide an important basis for the popularization and application of fragrant japonica hybrid rice.
作者
程灿
杨佳
周继华
牛付安
胡雪娇
涂荣剑
罗忠永
王新其
曹黎明
储黄伟
Cheng Can;Yang Jia;Zhou Jihua;Niu Fuan;Hu Xuejiao;Tu Rongjian;Luo Zhongyong;Wang Xinqi;Cao Liming;Chu Huangwei(Institute of Crop Breeding and Cultivation,Shanghai Academy of Agricultural Sciences,Shanghai,201403;College of Food Sciences and Technology,Shanghai Ocean University,Shanghai,201306;School of Agricultural Sciences,Jiangxi Agricultural University,Nanchang,330000)
出处
《分子植物育种》
CAS
CSCD
北大核心
2018年第17期5653-5659,共7页
Molecular Plant Breeding
基金
上海市水稻产业技术体系建设(沪农科产字(2017)第3号)
上海市科技兴农重点攻关项目(沪农科攻字(2014)第7-1-2号)
上海市种业发展项目(沪农科种字(2016)第1-3)共同资助
关键词
水稻
香味基因
分子标记
杂交粳稻
Rice
Fragrant gene
Molecular marker
Japonica hybrid rice
