摘要
试验采用水浴提取法提取七味地藿颗粒中的多糖,通过苯酚-硫酸显色体系运用紫外分光光度法对该方剂中多糖的含量进行测定,分析提取时间、提取次数、提取温度和料液比等因素的影响,并在此基础上进行正交试验,优化提取工艺参数。结果表明:当料液比为1∶25,提取时间为2 h,提取温度为90℃,提取次数为3次时,七味地藿颗粒多糖提取效果最好,提取率为28.01%。该优化工艺条件稳定、可靠,可作为工业化生产七味地藿颗粒的试验依据。
The study on Extraction of Polysaccharide from Qi Wei Di Huo Granule in the water bath to herb extraction method by phenol sulfuric acid chromogenic system using UV spectrophotometry on the prescription of polysaccharide content determination,analysis of extraction time,extraction times,extraction temperature and ratio of material to liquid,and on this basis by orthogonal experiment,optimization of extraction parameters.The results show that when the solid-liquid ratio was 1∶25,extraction time 2 h,extraction temperature of 90℃,2 times of extraction,Qi Wei Di Huo Granule to epimedium polysaccharide extract had the best effect,the extraction rate was 28.01%.The optimum technological conditions of stable and reliable theoretical basis,can produce seven flavor herb granules as industrialization.
作者
杨耀森
褚秀玲
YANG Yao-sen;CHU Xiu-ling(Agricultural College of Liaocheng University,Liaocheng,Shandong 252059,China)
出处
《中国兽药杂志》
2018年第8期44-49,共6页
Chinese Journal of Veterinary Drug
基金
山东省自然科学基金(ZR2013CL010)
关键词
七味地藿颗粒
多糖
水浴提取
正交设计
Qi Wei Di Huo Granule
polysaeeharide
extraction
orthogonal design
