摘要
骨髓间充质干细胞是一类存在于骨髓中的未分化细胞,而目前骨髓间充质干细胞的研究主要集中在哺乳动物,对于禽类骨髓间充质干细胞的研究较少,因此,介绍了禽类骨髓间充质干细胞的来源、分离纯化、表面标志物及多向分化潜能的最新研究进展。研究结果表明禽类BM-MSCs的来源主要是鸡和鸡胚期骨髓,分离纯化方法包括全骨髓贴壁法、密度梯度离心法和贴壁培养法,其表面标志物表达与否不尽相同且具有多向分化潜能,禽类品种、年龄、胚胎期、分离方法等不同使分离所得BM-MSCs在表面标志物和分化潜能方面存在差异。研究结果可以为禽类发育生物学及其用于再生医学的研究提供参考。
Bone marrow-derived mesenchymal stem cells （ BM-MSCs） are a kind of undifferentiatedcells that exist in bone marrow. At present BM-MSCs research mainly focuses on mstudies on BM-MSCs in poultry. This paper introduced the latest researches ofication ,surface markers and multidirectional differentiation potential of avian BM-MSCs. It showed that themain sources of poultry BM-MSCs are bone marrow from chicken and chicken embryo,and tand purification methods include whole bone marrowadherent method,density gradient centrifugation methodand adherent culture method. The surface mariners expression is diferent and shows mutiation potential. the differences in poultry breed,age, embryonic period and separation methods make theseparated BM-MSCs surface markers and differentiation potential show great variances. Tprovid the foundation for the research of poultry developmental biology and regenerative medicine.
作者
何琳琳
HE Lin-lin(Institute of Bioscience and Engineering,Vitamin D Research Institute,Shaanxi University of Technology,Hanzhong 723000,China)
出处
《陕西理工大学学报（自然科学版）》
2018年第4期66-69,79,共5页
Journal of Shaanxi University of Technology:Natural Science Edition
基金
陕西省科技厅自然科学基础研究计划项目(2018JM3033)
陕西省教育厅专项科研计划项目(2013JK0722)
陕西理工大学校级人才启动项目(SLGQD13(2)-26)
关键词
禽类
骨髓间充质干细胞
研究进展
poultry
bone marrow-derived mesenchymal stem cells （ BM-MSCs）
research progress
