摘要
黄精属Polygonatum为百合科Liliaceae多年生草本植物,在食品、医药等领域具有广阔的应用前景。该研究通过简单重复序列分子标记（simple sequence repeat,SSR）分析探讨了黄精属6种植物的遗传差异性和群体结构。从我国14个省38个县市共收集60份材料黄精属种质资源,利用49对SSR引物进行遗传差异性和群体结构的分析,结果显示：49对SSR引物共检测到211个等位基因,平均每个标记扩增得到4.306 1个等位基因,变化范围为2~10个;多态性信息含量的变异范围0.031 7~0.731 8,平均为0.309 6;聚类结果分析显示,60份黄精属种质材料在遗传距离阈值为0.26时可聚为4大类,其中大部分具有亲缘关系的同种材料聚为一类,但有6份材料并没有和同种的其他材料聚在一起,显现出种间交叉及地理分布交叉现象。4个地区来源群体间的遗传多样性指数从大到小排列依次为西部地区〉华中地区〉华南地区〉华东地区。群体结构分析表明,当K为4时,60份材料可分为4个类群,且与SSR聚类分析结果有一定的相似性。以上结果表明,黄精属植物遗传多样性丰富,变异幅度大,种的界限相对模糊,互生叶系与轮生叶系的某些植物存在差异减小的现象;西部地区的遗传多样性较其他地区高,可能是我国黄精属植物的起源中心。
Polygonatum is a genus of the perennial herb family Liliaceae,with great potential in food,medicine and other field. In this study,genetic diversity and cluster structure analysis of 6 species in Polygonatum were investigated the molecular marker technique of simple sequence repeat（SSR）. A total of 49 SSR makers were used to study genetic diversity and population structure within 60 germplasm resources which obtained from 38 counties and cities in 14 provinces of China. A total of 211 alleles were identified and the number of alleles ranged from 2 to 10,with an average of 4. 306 1 alleles per SSR. The range of polymorphism information content（PIC） varied from 0. 731 8 to 0. 031 7,with the average of 0. 309 6. The cluster analysis classified 60 germplasm resources into four defined groups at the genetic distance value of 0. 26,among which most species with relatives were clustered into the same group. Extraordinarily,there were 6 germplasm resources clustered into other species,indicating that the classification of inter-genus and geographical distribution was crossed in Polygonatum. The genetic diversity index of the 4 geographical populations from high to low was：Western region,Central China,Southern China,Eastern China. The population structure analysis,also indicating divided the entire collection into four groups,which was similar to the assignment pattern of cluster structure analysis. These results suggested that the Polygonatum germplasm resources used in this study is rich in relatively high genetic diversity with large variation range,relatively fuzzy boundaries of species. It appeared the phenomenon that there is a difference decreases between the alternate leaf system and the rotation leaf system. The genetic diversity in the western region was higher than that in other regions,and the western region may be the origin center of the genus Polygonatum.
作者
朱巧
邓欣
张树冰
梅时勇
陈小军
张冀芳
肖清明
黎宇
ZHU Qiao;DENG Xin;ZHANG Shu-bing;MEI Shi-yong;CHEN Xiao-jun;ZHANG Ji-fang;XIAO Qing-ming;LI Yu(Key Laboratory of Risk Assessment for Plant Fiber Crops,Ministry of Agriculture,Institute of Bast Fiber Crops Chinese Academy of Agricultural Sciences,Changsha 410205,China;School of Life Sciences,Central South University,Changsha 410013,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2018年第14期2935-2943,共9页
China Journal of Chinese Materia Medica
基金
中国农业科学院科技创新工程项目(ASTIP-IBFC05)
关键词
黄精属
种质材料
SSR分子标记
遗传差异
群体结构
Polygonatum
germplasm resources
SSR molecular marker
genetic diversity
population structure
