摘要
目的:探讨滋补脾阴方药防治轻度认知功能障碍的物质基础与作用机制。方法:利用整合药理学平台,检索查询滋补脾阴方药12味中药的活性成分、药物靶标,以及轻度认知功能障碍的疾病靶标,共同构建"滋补脾阴方药-中药材-化学成分-核心靶标-关键通路"的可视化网络并进行网络分析。结果:平台筛选出滋补脾阴方药687种活性成分,主要为苷类、糖类、醇类;及595个治疗轻度认知功能障碍的关键靶标,包括羟酰辅酶A脱氢酶三功能多酶复合物亚基α(HADHA)、羟酰-Co A脱氢酶(HADH)、NAD(P)依赖类固醇脱氢酶样(NSDHL)等。GO和KEGG富集分析显示这些关键靶标主要定位于细胞质、线粒体,最常见的分子功能是ATP结合、蛋白结合等,并参与嘌呤代谢、核苷酸代谢、碳代谢、碳水化合物代谢等通路。结论:通过该平台能够预测出滋补脾阴方药防治轻度认知功能障碍的关键靶标及参与的相关通路,为进一步深入揭示其作用机制奠定了良好基础。
To investigate the material foundation and mechanism of Zi Bu Pi Yin Recipe in the prevention and treatment of mild cognitive impairment. The integrated pharmacology platform was used to study the active ingredients, drug targets,and disease targets of 12 Chinese traditional medicines of Zi Bu Pi Yin Recipe, and to construct the target of＂Zi Bu Pi Yin Recipe-Chinese herbal medicine-chemical composition-core Target-the key path ＂of the visual network and network analysis. Results：The platform screened 687 kinds of active ingredients of Zi Bu Pi Yin Recipe including glycosides,sugars and alcohols. and 595 key targets for treating mild cognitive impairment, Including HADHA, HADH, NAD（P）-dependent steroid dehydrogenase-like（NSDHL）, and et al. The enrichment analysis of GO and KEGG showed that these key targets were mainly localized in the cytoplasm and mitochondria. The most common molecular functions were ATP binding and protein binding, and participated in purine metabolism, nucleotide metabolism, carbon metabolism pathway,Carbohydrate metabolism and so on. The platform could predict the key target of Zi Bu Pi Yin Recipe in preventing and treating Mild Cognitive Impairment and its related pathways, which lay a good foundation for further revealing its mechanism.
作者
周雯
战丽彬
Zhou Wen;Zhan Libin(Basic Medical College,Modern Research Laboratory of Spleen Visceral Manifestations Theory/Nanfing University of Chinese Medicine,Nanjing 210023,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2018年第4期488-495,共8页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委员会重点项目(81730111):基于肠道菌群"从脾论治"疾病易感痰湿体质的中医治未病的生物学基础研究
负责人:战丽彬
国家自然科学基金委员会重点项目(81230084):情志所致糖尿病认知功能障碍的机理及滋补脾阴法防治基础研究
负责人:战丽彬
江苏省教育厅高校优势学科建设工程资助项目:中医学学科建设
负责人:方祝元
关键词
整合药理学
滋补脾阴方药
轻度认知功能障碍
物质基础
作用机制
Integrated pharmacology
Zibu piyin recipe
mild cognitive impairment
material basis
functional mechanism
