摘要
为了分析我国新出现的Ⅰ类新城疫病毒的分布和分子特性,对2016年分离到的4株Ⅰ类新城疫病毒进行F基因序列分析和遗传进化分析。结果显示:4株Ⅰ类新城疫病毒F基因编码长度均为1 662nt,共编码553个氨基酸,分离株之间氨基酸相似性为97.9%~100%。4株分离株F蛋白裂解位点的氨基酸组成均为112 ERQERL117,符合新城疫病毒弱毒株的分子特征。F基因高变区(47—420nt)遗传进化分析结果显示这4株病毒为国内新出现的基因型,利用F基因编码区全长序列构建系统进化树显示4株病毒属于基因1c亚型,与北美分离株高度同源,而与国内基因1c亚型分离株(Duck/Guangxi/1261/2015)相似性仅为95.5%~97%。监测数据表明这种新出现的Ⅰ类新城疫病毒具有在国内进一步流行和扩散的风险。
In order to understand the distribution and molecular characteristics of new emerged classⅠ Newcastle disease virus in China.We carried out the Fgene sequence analysis and phylogenetic analysis of 4 viruses isolated in 2016.Results were as follows：The length of complete F gene of 4 NDV isolates were 1 662 nt,encoding 553 amino acids,and the amino acid identity among all isolates was 97.9%-100%.The amino acid motif at F protein cleavage site of the 4 isolates was 112 ERQERL117,which was consistent with that of lentogenic Newcastle disease virus.The phylogenetic analysis of Fgene（47-420 nt）showed that all 4 isolates were newly emerging NDVs in China.Phylogenetic tree constructed by the complete Fgenes showed 4 ClassⅠ viruses belonged to sub-genotype 1 c,which showed a high level of similarity with isolates obtained from North America,and had the identity of 95.5%-97% with the sub-genotype 1 cvirus（Duck/Guan-gxi/1261/2015）isolated in China.Our results indicated that the new emerged class Ⅰ NDVs had the ability to circulate and spread in China.
作者
魏润宇
罗瑶瑶
吕艳
王静静
郑东霞
赵云玲
刘文博
刘华雷
王志亮
WEI Run-yu;LUO Yao-yao;LUYan;WANG Jing-jing;ZHENG Dong-xia;ZHAO Yun-ling;LIU Wen-bo;LIU Hua-lei;WANG Zhi-liang(College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China;China Animal Health and Epidemiology Center,Qingdao 266032,China;Qingdao Agricultural University,Qingdao 266109,China;A Project Funded by the Priority Academic Program Development of J iangsu Higher Education Institutions,Yangzhou 225009,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2018年第8期1781-1788,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家重点研发计划(2016YFD0500800)
公益性行业(农业)科研专项(201303033)
江苏高校优势学科建设工程资助项目(PAPD)
关键词
Ⅰ类新城疫病毒
分子特征
遗传进化分析
Class I Newcastle disease virus
molecular characteristics
phylogenetic analysis
