摘要
旨在探求不同批次玉米及其副产品(玉米皮、玉米胚芽(带壳)、玉米胚芽饼、玉米蛋白粉)常规营养价值和脂质分子结构及其两者之间是否存在相关性。试验采用傅里叶变换红外(FTIR)光谱技术和常规营养分析技术,分析样本的脂质分子功能团结构与常规营养成分。这些振动谱带的功能团特征参数包括CH_3反对称伸缩(峰高位置,ca.2 942~2 934cm^(-1))、CH_2反对称伸缩(峰高位置,ca.2 905~2 903cm^(-1))、CH_3对称伸缩(峰高位置,ca.2 866~2 850cm^(-1))、CH_2对称伸缩(峰高位置,ca.2 834~2 832cm^(-1))的光谱区域及峰高位置,不饱和脂质(ULB)伸缩(峰高位置,ca.2 987~2 986cm^(-1))和脂质酯的羰基(LECC)伸缩(峰高位置,ca.1 734~1 730cm^(-1))的峰高、峰面积以及它们之间的比值。本次试验还通过聚类分析(CLA)和主成分分析(PCA)来识别分子光谱差异。试验结果表明:1)常规营养成分分析表明,玉米与其副产品之间的营养成分存在显著差异(P<0.05),不同批次间也存在显著差异(P<0.05),而且两因素之间存在显著的互作效应(P<0.05)。2)光谱特征分析显示,玉米与其副产品之间的光谱分子功能团结构存在显著差异(P<0.05),不同批次间LECC峰高值和CH_2反对称/CH_2对称伸缩峰高比值无显著差异(P>0.05),并且除CH功能团部分,其他光谱参数间均存在显著的互作效应(P<0.05)。3)聚类分析(CLA)和主成分分析(PCA)结果表明,不同批次间的脂质光谱分子结构不能完全分开,而玉米胚芽与玉米胚芽饼在CH_3和CH_2不对称和对称伸缩区域(ca.2 984~2 781cm^(-1))、ULB光谱区域(ca.3 016~2 971cm^(-1))和LECC光谱区域(ca.1 826~1 693cm^(-1))中均有完全不同的结构组成,然而,其他饲料样品间在脂质生物聚合物构象中仍有相似之处。4)大多数脂质分子功能团参数与其化学成分粗脂肪(EE)间存在着显著的相关关系(P<0.05)。综上所述,玉米及其副产品的脂质分子结构与常规营养特性间
The objective of this study was to reveal the basic chemical profile and lipid molecular structural features,as well the relationship between molecular structural spectral parameters and nutritional values in different batches of corn and its by-products（corn gluten feed,corn germ,corn germ cake and corn gluten meal）.This study was conducted to characterize the lipid nutri-tive values and lipid structure spectral features in different batches of samples using the conventional chemical analysis and Fourier transform infrared spectroscopy（FTIR）techniques,respectively.The spectroscopy parameters included spectral regions and peak heights of lipid CH3 asymmetric（central peak ca.2 942-2 934 cm^-1）,CH2 asymmetric（central peak ca.2 905-2 903 cm^-1）,CH3 symmetric（central peak ca.2 866-2 850 cm^-1）and CH2 symmetric（central peak ca.2 834-2 832 cm^-1）functional groups,unsaturation lipid bands group（CH attached to C-C）（central peak ca.2 987-2 986 cm^-1）,lipid ester C=O carbonyl group（central peak ca.1 734-1 730 cm^-1）as well as the ratios among them.Multivariate spectral analyses were conducted by the hierarchical cluster analysis（CLA）and the principal component analysis（PCA）for all batches of corn and its co-product samples to investigate the molecular spectral differences.The results showed that：1）The significant differences were found in the lipid nutritive values among different batches and between corn and its by-products（P〈0.05）,and there was significant interaction was found between the two factors（P〈0.05）;2）The result from FTIR spectroscopy analysis showed a significant interaction among the spectrum parameters except for CH functional groups（P〈0.05）.And a significant difference was found in lipid spectral molecular structures between corn and its by-products（P〈0.05）.However,no significant difference was found in the height of LECC and the height ratio of CH2 asymmetric to CH2 symmetric among different batches（P 0.05）;3）The results of CLA
作者
孙凯晶
李昕
刘帅
王馨影
张广宁
杨金山
辛杭书
张永根
SUN Kai-jing;LI Xin;LIU Shuai;WANG YANG Jin-shan;XIN Hang-shu;Xin-ying;ZHANG Guang-ning;ZH(College of Animal Science and Technology ANG Yong-gen Northeast Agricultural University,Harbin 150030,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2018年第8期1674-1686,共13页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家奶牛产业技术体系(CARS-36)
黑龙江省科学基金项目计划任务书(LC2017011)
