摘要
目的探讨重症与非重症、重症急性期与恢复期腺病毒肺炎患儿血液中的T细胞亚群变化及临床意义。方法将符合肺炎标准且咽拭子荧光PCR检测腺病毒抗原阳性的60例患儿分为重症肺炎组32例和非重症肺炎组28例,采用流式细胞术检测两组患儿的T细胞亚群CD3、CD4、CD8、CD4/CD8比值并进行两组间对照;重症肺炎组同时动态检测急性期和恢复期的T细胞亚群值并作比较分析。结果重症肺炎组CD3、CD4、CD8及CD4/CD8比值均低于非重症肺炎组,差异均有统计学意义(P<0.05或0.01)。重症肺炎患儿恢复期CD3、CD4、CD8及CD4/CD8比值均高于急性期,差异均有统计学意义(P<0.05或0.01)。结论腺病毒所致重症肺炎发生时,患儿外周血T淋巴细胞亚群CD3、CD4、CD8及CD4/CD8的比值均降低,提示机体的细胞免疫功能下降。经治疗,恢复期外周血T淋巴细胞亚群CD3、CD4、CD8及CD4/CD8的比值均升高,提示免疫紊乱重新达到平衡。
Objective To investigate the changes of T lymphocyte subsets in children with severe adenoviral pneumonia and its clinical significance. Methods Sixty children with adenoviral pneumonia confirmed by fluorescence PCR tests were enrolled in the study, including 32 cases of severe disease（severe group） and 28 cases with moderate disease（non-severe group）. The T cell subsets CD3, CD4, CD8 and CD4/CD8 were measured by flow cytometry in two groups and at the acute phase and recovery phase in severe group. Results The levels of CD3, CD4, CD8 and CD4/CD8 in severe group were significantly lower than those in non-severe group（P〈0.05 or 0.01）.The levels of T lymphocyte subsets at acute phase were lower than those at recovery phase in the severe group（P〈0.05 or 0.01）. Conclusion The levels of T lymphocyte subsets in severe adenoviral pneumonia are significantly decreased, and they return to normal levels after successful treatment.
作者
郑丽丽
许航燕
应旦红
ZHENG Lili;XU Hangyan;YING Danhong(Department of Paediatrics,Dongyang People＇ s Hospital,Jinhua 322100,China)
出处
《浙江医学》
CAS
2018年第15期1702-1704,共3页
Zhejiang Medical Journal
基金
浙江省卫计委医药卫生科技项目(2015KYB421)
