摘要
本试验旨在研究维生素A对奶牛乳腺上皮细胞(BMECs)内乳脂和乳蛋白合成相关基因表达的影响。采用单因素完全随机试验设计,将第3代BMECs随机分为6个处理,每个处理6个重复,使用无血清培养基饥饿24 h后,采用维生素A浓度分别为0(对照)、0.05、0.10、0.20、1.00和2.00μg/m L的培养基培养24 h。结果表明:与对照组相比,1.00、2.00μg/m L维生素A可以显著提高相对增殖率以及甘油三酯(TG)含量(P<0.05);维生素A能显著提高乳脂合成相关基因过氧化酶体增殖物激活受体γ(PPARG,0.05、0.10、0.20、1.00和2.00μg/m L)、固醇调节元件结合蛋白1(SREBP1,0.05、0.10μg/m L)、硬脂酰辅酶A去饱和酶(SCD,0.05、0.10μg/m L)的基因表达量(P<0.05);维生素A也能显著提高乳蛋白合成相关基因信号转导转录激活因子5(STAT5,0.20μg/m L)、αs1-酪蛋白(CSN1S1,0.05和0.10μg/m L)、κ-酪蛋白(CSN3,0.10μg/m L)基因表达量(P<0.05);维生素A也能显著提高乳脂合成相关酶乙酰辅酶A羧化酶(ACACA)活性(0.05、0.10、0.20和1.00μg/m L)(P<0.05)。结果提示,维生素A对BM ECs内乳脂、乳蛋白合成相关基因表达的促进效果呈浓度依赖性,以0.10μg/m L维生素A效果较好。
This experiment was conducted to investigate the effects of vitamin A on gene expresions related to milk fat and protein synthesis in bovine mammary epithelial cells （BMECs）. The experiment was designed as a single factor randomized trial, and the 3rd generation BMECs were randomly divided into 6 treatments with 6 replicates per group. After cultured in culture medium without serum for 24 h, cells in different treatments were cultured in culture medium supplemented with 0（control）, 0.05, 0.10, 0.20, 1.00 and 2.00 μg/mL vitamin A for 24 h, respectively. The results showed that compared with the control group, 1.00 and 2.00 μg/mL vitamin A could significantly increase relative growth rate and triacylglycerols （TG） content （ P 〈0.05）; vitamin A could significantly up-regulate expression levels of genes related to milk fat synthesis, such as peroxisome proliferator-activated receptor γ （ PPARG , 0.05, 0.10, 0.20, 1.00 and 2.00 μg/mL）, sterol regulatory element-binding protein 1 （ SREBP1 , 0.05 and 0.10 μg/mL）, stearoyl-CoA desaturase （ SCD , 0.05 and 0.10 μg/mL ） （ P 〈0.05）; vitamin A could significantly up-regulate expression levels of genes related to milk protein synthesis, such as signal transducers and activators of transcription 5 （ STAT5 , 0.20 μg/mL）, α-casein （ CSN1S1 , 0.05 and 0.10 μg/mL） and κ-casein （ CSN3 , 0.10 μg/mL） （ P 〈0.05）; vitamin A could significantly increase acetylcoenzyme A （ ACACA ） activity （0.05, 0.10, 0.20 and 1.00 μg/mL） （ P 〈0.05）, which was related to milk fat synthesis. In conclusion, vitamin A has concentration-dependent effects on expressions of genes related to milk fat and protein synthesis in BMECs, and the promotion effects of 0.10 μg/mL vitamin A are better.
作者
苏芮
刘阳
闫素梅
史彬林
赵艳丽
石惠宇
SU Rui ,LIU Yang ,YAN Sumei, SHI Binlin ,ZHAO Yanli ,SHI Huiyu(College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, Chin)
出处
《动物营养学报》
CAS
CSCD
北大核心
2018年第8期3151-3158,共8页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金(31160466)
关键词
维生素A
乳脂
乳蛋白
基因表达
vitamin A
milk fat
milk protein
gene expression
