摘要
为考察铀污染对土壤真菌群落结构的影响,通过室内实验模拟不同浓度(2、5、10、20、50、100 mg·kg^(-1))铀污染土壤环境,以未处理土样作为空白对照。结合Illumina Mi Seq高通量测序技术和生物信息学分析方法,测定了不同浓度铀处理下土壤真菌的多样性及群落结构。研究结果表明,铀处理对真菌多样性有重要影响,铀浓度为20 mg·kg^(-1)时,真菌多样性最低。在门水平上,主要以Zygomycota为主,其所占比例为33.46%~73.36%;在属水平上,主要以Mortierella为主,其所占比例为33.46%~73.30%。主成分分析结果表明,土壤受到铀胁迫后,其真菌群落结构与对照组有明显差异。利用相关性分析得到10种对铀具有耐性的真菌,分别是Pseudeurotium、Glomus和Cylindrocarpon等。该研究结果可为评估和修复铀污染生态环境提供理论依据。
In order to investigate the effect of uranium pollution on the community structure of soil fungi, in this study, indoor pollution by uranium of the soil environment（2, 5, 10, 20, 50 mg·kg^（-1） and 100 mg·kg^（-1））was simulated, and untreated soil samples were blank controls. Using a combination of Illumina Mi Seq high-throughput sequencing and bioinformatics analysis method, the diversity and community structure of soil fungi under the different concentrations of uranium were determined. The results showed that uranium had an important influence on fungal diversity. When the uranium concentration was 20 mg·kg^（-1）, the fungal diversity was the lowest. At the phylum level, the dominant strains were from the Zygomycota, accounting for 33.46%-73.36%, with Mortierella the dominant genus, accounting for 33.46%-73.30%. Principal component analysis showed that the fungal community structure of the soil under uranium stress was significantly different from that of the control group. Ten species of uranium-resistant fungi were found via correlation analysis, such as Pseudeurotium, Glomus, and Cylindrocarpon. The results of this study can provide a theoretical basis for evaluating and remediating ecological environments polluted by uranium.
作者
肖诗琦
宋收
陈晓明
戚鑫
张祥辉
田甲
黄森
董发勤
XIAO Shi-qi;SONG Shou;CHEN Xiao-ming;QI Xin;ZHANG Xiang-hui;TIAN Jia;HUANG Sen;DONG Fa-qin(College of Life Science and Engineering,Southwest University of Science and Technology,Mianyang 621010,China;Deibnse Key Labo-ratory,Southwest University of Science and Technology,Mianyang 621010,China)
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2018年第8期1698-1704,共7页
Journal of Agro-Environment Science
基金
国家核设施退役及放射性废物治理科研重点项目(16ZG6101)
国家重点基础研究发展计划项目(2014CB846003)
四川省科技厅项目(18YYJC0927)~~
关键词
铀污染
真菌群落结构
高通量测序
铀耐性菌
uranium pollution
tungal conmmunity structure
high-throughput sequencing
uranium-resistant fungi
