摘要
目的:探讨维生素E(Vit E)预处理对心肌缺血/再灌注损伤(MI/RI)大鼠血流动力学的影响。方法:选取健康SD大鼠60只,按照随机数字表法,随机分为Sham组、MI/RI组、Vit E组,每组各20只。Vit E组在造模前两周开始,肌注Vit E注射液4.5mg·kg-1·d-1,另两组术前肌注生理盐水4.5 mg·kg-1·d-1,均连续给药2周。MI/RI组、Vit E组在平左心耳下缘下2 mm处结扎左冠脉前降支(LAD),30 min后松解结扣再灌注120 min,复制MI/RI模型;Sham组只用丝线穿过LAD,不结扎。结果:与MI/RI组比较,Vit E组大鼠超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性升高,丙二醛(MDA)含量降低,差异均有统计学意义(P<0.05);MI/RI组肌钙蛋白Ⅰ(c Tn I)(ng·mL-1)、心钠素(ANP)(ng·L-1)分别为(6.57±2.45)、(282.79±24.87),与Vit E组(4.51±2.06)、(254.68±20.84)比较,后者均降低(P<0.05);t3时,Vit E组心率(HR)(次·min-1)、左心室收缩末期压力(LVESP)(mm Hg)、左心室舒张末期压力(LVEDP)(mm Hg)、心室内最大压力上升速率(+dp/dt max)(mm Hg·s-1)、心室内最大压力下降速率(-dp/dt max)(mm Hg·s-1)分别为(331.9±30.1)、(91.7±15.1)、(1.1±1.8)、(525±195)、(399±169),与MI/RI组(308.9±32.8)、(77.1±12.5)、(3.3±2.7)、(319±140)、(336±197)比较均有所改善,两者差异均有统计学意义(P<0.05)。结论:Vit E预处理可以对抗心肌缺血/再灌注损伤,改善MI/RI大鼠血流动力学指标,从而保护MI/RI心肌细胞的结构和舒缩功能。
Objective：To explore the impact of vitamin E（ Vit E） preconditioning on the hemodynanlies of rats with myocardial isehe-mia/reperfusion injury-（MI/MR）. Methods： 60 healthy SD rats were selected and divided into the Sham group, MI/MR group, and Vit E group in accordance with the method of random number table, with 20 rats in each group. Two weeks before the building of models, the Vit E group started to intramuscular inject with Vit E injection 4. 5 mg · kg ^-1· d ^-1, the other two groups started to intranmseular inject with the normal saline 4. 5 mg· kg^-1· d ^-1, and continued 2 weeks. The left anterior descending branch of coronary artery was occluded at 2 mnl under the bottom edge of the fiat left atrial appendage for MI/RI group and Vit E group, loosen the knot after 30 min and fill with another 120 min, copying the MI/RI model. Use the silk wire to get through LAD for Sham group, it should not be occluded. Results： Compared with MI/RI group, the activity of SOD, GSH-Px of the rats in Vit E group was apparently increased, the content of MDA was deereased（P 〈 0. 05）. eTnI（ng · mL^-1 ）, ANP（ ng · L^-1 ） of MI/RI group were （6. 57 ±2.45）, （282. 79 ±24. 87） respec-tively, compared with （4. 51 ± 2.06）, （254. 68 ± 20. 84） for Vit E group, the latter one was decreased （P 〈 0. 05）. At t3, HR （time. min^-1）, LVESP（mmHg）, LVEDP（mmHg）, ＋dp/dt max（mmHg · s^-1）, -dp/dt max（mmHg · s^-1） of Vit E group are （331.9 ± 30.1）, （91.7 -＋ 15.1）, （1.1 ± 1.8）, （525±195）, （399 ± 169） respectively, compared with （308.9 ± 32.8）,（77. 1 ± 12. 5）, （3.3 ±2. 7）, （319 ± 140）, （336 ± 197） of MI/RI group, the index were improved, the difference was statistically sig-nificant（ P 〈 0. 05 ）. Conclusion：Vit E preconditioning may improve the index of hemodynamics of rats with myocardial ischemia/reperfu-sion injury to protect the structure and systolic and diastolic function of the cardiocyte with MI/RI.
作者
陈玲玲
彭贵海
王崇全
CHEN Lingling;PENG Guihai;WANG Chongquan(Department of Cardiology,Taihe Hospital Affiliated to Hubei University of Medicine,Hubei Shiyan 442000,China)
出处
《中国医药导刊》
2018年第6期351-355,共5页
Chinese Journal of Medicinal Guide
基金
2016年十堰市科学技术研究与开发计划项目(项目编号:SYKJ20160016
项目名称:维生素E预处理对心肌缺血/再灌注损伤大鼠血流动力学的影响)
关键词
心肌缺血/再灌注损伤
氧化应激
维生素E
血流动力学
药物预适应
Myocardial ischemia/repeffusion injury-
Oxidative stress
Vitamin E
Hemodynanlics
Pharmacologic preconditioning
