摘要
目的:构建一种自控式缓释系统,能持续、有效地控制大鼠体内异常增高的铬、钴离子。方法:制备负载乙二胺四乙酸(ethylene diamine tetraacetic acid,EDTA)的缓释系统。通过膝关节腔注射铬钴钼(Co Cr Mo)纳米微粒悬液构建体内铬、钴离子升高的大鼠模型。将大鼠模型随机分为4组,分别经腹腔注射生理盐水(生理盐水组)、EDTA溶液(EDTA组)、二氧化硅微球空载体溶液(空载组)及载有EDTA的自控式缓释系统溶液(载药组)。在此过程中继续注射纳米颗粒悬液,观察大鼠血清铬、钴离子浓度的变化。结果:在经过药物治疗之后,短期内EDTA组和载药组金属离子浓度均显著降低。而随着时间延长,EDTA组大鼠血清金属离子浓度迅速回升,载药组则保持相对正常,差异具有统计学意义。结论:这种自控式缓释系统能持续有效降低实验动物体内异常增高的金属离子,为治疗金对金人工关节置换术后金属离子的异常增高提供选择。
Objective：To construct a sustained-release system to control the abnormally increase of chromium and cobalt ion concentration persistently and effectively in rats. Methods：First,a sustained-release system loaded with ethylene diamine tetraacetic acid（EDTA）was constructed. Then,the rat models were established by intra-articular injection of Co Cr Mo nanoparticles. Afterwards,the rat models were randomly divided into 4 groups,injected intraperitoneally with normal saline,EDTA,silica microsphere empty carrier solution and EDTA-loaded system solution,respectively,during which,the rats were injected with Co Cr Mo nanoparticles as before. The blood chromium and cobalt ion concentrations were measured. Results：After the therapy,the concentration of metal ions in groups receiving EDTA and EDTA-loaded system solution decreased significantly in the short run. However,as the time goes,the concentration of metal ions in group injected with EDTA increased and with EDTA-loaded system solution,stayed low,relatively,there was a statistically significant difference between these 2 groups. Conclusion：This sustained release system can effectively reduce the continuous increase of metal ions in the serum of rats,which can offer an alternative treatment for the abnormally increased metal ion after arthroplasties.
作者
陈桂文
葛雨庆
刘杨
刘锋
Chen Guiwen;Ge Yuqing;Liu Yang;Liu Feng(Department of Orthopedics,the First Affiliated Hospital of NMU,Nanjing 210029,China)
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2018年第7期940-944,982,共6页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金(81572136)
关键词
缓释系统
金属对金属
离子浓度
sustained-release system
metal-on-metal
metal ion alonormal increase
