摘要
电喷雾是质谱分析多肽和蛋白质等生物分子时最常用的离子化技术。电喷雾产生的多肽和蛋白质离子通常带有多电荷,从而产生多个谱峰,导致谱图复杂。通过气相离子/离子间的质子转移反应可以有效调节经电喷雾离子化后蛋白和多肽等生物大分子的电荷价态,简化谱图,对于复杂蛋白和多肽生物样品的分析具有重要意义。本研究通过研制的一套双极性离子阱质谱装置,利用阱内正、负离子间的质子转移反应,实现了多肽离子的电荷调控。以氧化型谷胱甘肽、缩宫素、强啡肽分子作为典型样品对方法的检测效果进行了验证。结果表明,此方法可去除多肽正离子中多余的正电荷,当注入足够的负离子进行反应时,可以将带多个电荷的多肽阳离子的价态降至最低,从而有效简化谱图。
Electrospray ionization is most commonly used in mass spectrometry for analysis of biologicalmolecules such as peptides and proteins. However, peptides and proteins ions produced by electrosprayionization usually have multiple charges, and produce multiple spectrum peaks, making the mass spectrumcomplicated. Gas phase proton transfer ion / ion reaction can effectively regulate the charge states of peptidesand proteins ions after electrospray ionization, simplifying the spectrogram, and thus is significant to theanalysis of complex proteins and peptides samples. In this study, a dual-polarity linear ion trap （LIT） massspectrometer was used to control the charge state of peptide ions by proton transfer ion / ion reaction. Thedetection effect of the method was verified by using glutathione （ oxidation type）, oxytocin and dynorphin astypical samples. The results showed that this method could remove excess charges in the positive ions. Wheninjecting enough negative ions for eaction, peptide ions with multiple charge valence state could be reduced tothe minimum, therefore simplify the spectrogram effectively.
作者
叶似剑
龚晓云
李明
刘大军
江游
方向
YE Si-Jian;GONG Xiao-Yun;LI Ming;LIU Da-Jun;JIANG You＊;FANG Xiang(Changchun University of Science and Technology,Department of Chemistry ＆ Environmental Engineering,Changchun 130022,China;National Institute of Metrology,Beijing 100029,China)
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2018年第7期1084-1088,共5页
Chinese Journal of Analytical Chemistry
基金
本文系国家重点研发计划项目(No.2016YFF0200504)和国家自然科学基金项目(No.21605135)资助
关键词
质子转移反应
多肽
质谱
正负离子反应
Proton transfer reaction
Peptide
Mass spectrometry
Positive and negative ion / ion reaction
