摘要
目的探讨Wnt5a及骨形成蛋白7(BMP7)基因在先天性肛门直肠畸形中的表达。方法将已受孕的大鼠(35只)根据是否胃管注入乙烯硫脲(ETU)以及是否存在肛门直肠畸形分为对照组(12只)、ETU无畸形组(8只)和ETU畸形组(15只)。RT-PCR检测妊娠第13、14、15、16天大鼠直肠及泄殖腔Wnt5a和BMP7的mRNA表达;Western blot检测妊娠第16天大鼠直肠Wnt5a和BMP7的蛋白表达;高位畸形、低位畸性患者及非胃肠道疾病死亡,分为高位畸形组、低位畸形组和对照组,RT-PCR和Western blot分别检测直肠末端Wnt5a和BMP7的mRNA和蛋白表达。结果 ETU组大鼠的Wnt5a及BMP7基因的mRNA表达在4个时间点均显著低于对照组,差异均有显著统计学意义(P<0.01);ETU畸形组大鼠的Wnt5a和BMP7的蛋白表达显著低于对照组,差异均有显著统计学意义(P<0.01),而大鼠ETU无畸形组Wnt5a和BMP7的蛋白表达与对照组比较差异均无统计学意义(P>0.05);人高位畸形组大鼠的Wnt5a和BMP7的mRNA和蛋白表达均显著低于对照组,差异均有显著统计学意义(P<0.01),而人低位畸形组Wnt5a和BMP7的mRNA和蛋白表达与对照组比较差异均无统计学意义(P>0.05)。结论 Wnt5a和BMP7基因的表达下降可能与先天性肛门直肠畸形有关。
Objective To investigate the expression of Wnt5 a and BMP7 genes in congenital anorectal malformations. Methods The pregnant rats（ 35 rats） were divided into the control group（ 12 rats）,the ETU free group（ 8 rats） and the ETU malformed group（ 15 rats） according to whether injected with ETU and if there was anorectal malformation. The expression of Wnt5 a and BMP7 mRNA were detected on 13 d,14 d,15 d,and 16 d of gestation in rectum and cloaca of rats by RT-PCR. The expression of Wnt5 a and BMP7 protein on sixteenth day of pregnancy rats rectum were detected by Western blot. Patients with high malformation,low malformation,and non-gastrointestinal diseases were divided into high malformation group,low malformation group,and control group. RT-PCR and Western blot were used to detect the mRNA and protein expression of BMP7 and Wnt5 a. Results Wnt5 a and BMP7 gene mRNA expression in rat ETU group in the 4 time points were significantly lower than the control group（ P〈0. 01）. The expression of Wnt5 a and BMP7 protein in rat ETU abnormal group was significantly lower than the control group（ P〈0. 01）.The expression of Wnt5 a and BMP7 protein in rat ETU without abnormal and the control group had no significant difference（ P〈0. 05）. The mRNA and protein expression of Wnt5 a and BMP7 in human high deformity group were significantly lower than those of the control group（ P〈0. 01）. The mRNA and protein expression of Wnt5 a and BMP7 in human low malformation group and the control group had no significant difference（ P〈0. 05）.Conclusion The decreased expression of Wnt5 a and BMP7 genes may be associated with congenital anorectal malformations.
作者
李超
陈霞
LI Chao;CHEN Xia(Gastroen-terology,Shiyan Taihe Hospital;Shiyan Taihe Hospital Children＇s Medical Center, Shiyan Hubei 442000, China)
出处
《临床和实验医学杂志》
2018年第13期1381-1384,共4页
Journal of Clinical and Experimental Medicine
