摘要
目的比较两种麻醉方法用于恒河猴急性脑梗死模型的麻醉效果。方法选择拟行大脑中动脉M2段栓塞建立急性脑梗死模型的健康成年恒河猴16只,雄性,采用数字表法随机分为2组(n=8):丙泊酚组(P组)、丙泊酚+右美托咪定组(PD组)。所有恒河猴采用0.1 m L/kg氯胺酮及速眠新的混合液肌内注射进行基础麻醉。待动物入睡后行气管内插管,接呼吸机行机械通气,P组麻醉维持采用持续输注丙泊酚,PD组麻醉维持采用持续输注丙泊酚及右美托咪定,两组必要时均追加上述氯胺酮与速眠新的混合液。大脑中动脉M2段栓塞模型制备结束后,停止输注麻醉维持药物,待恒河猴恢复自主呼吸,行头部磁共振成像(magnetic resonance imaging,MRI)检查。记录术中血流动力学变化、自主呼吸恢复时间、建模术中追加氯胺酮与速眠新混合液容量、丙泊酚用量、麻醉合并症发生情况。结果 16只恒河猴全部存活超过24 h。实验中16只恒河猴未发生躁动、呼吸抑制、心律失常等严重合并症。血流动力学指标、体温、动脉血气分析均在正常范围。两组不同时刻血流动力学指标、体温、动脉血气分析比较差异无统计学意义(P>0.05)。全部恒河猴均在实验结束停药后30 min内恢复自主呼吸,两组自主呼吸恢复时间比较,差异无统计学意义(P>0.05)。PD组术中丙泊酚用量、术中追加氯胺酮与速眠新混合液容量低于P组(P<0.05)。结论两种麻醉方法用于恒河猴大脑中动脉M2段栓塞建立急性脑梗死模型麻醉效果良好,麻醉深度可控、安全性高。右美托咪定可减少建模术中丙泊酚、氯胺酮与速眠新混合液用量。
Objective To compare the effects of two anesthetic procedures on the establishment of a rhesus monkey model of acute cerebral infarction. Methods Sixteen male rhesus monkeys were randomized into 2 groups： propofol group（group P）,propofol ＋dexmedetomidine group（group PD）. Sumianxin injection combined with 0. 1 m L/kg ketamine was given before endotracheal intubation.After intubation,the animals received mechanical ventilation. In Group P,anesthesia was maintained with propofol infusion. In Group PD,anesthesia was maintained with propofol and dexmedetomidine infusion. Sumianxin-ketamine could be given if necessary to maintain adequate anesthesia depth. The infusion of anaesthetics was stopped after the establishment of model of the M2 segment of the middle cerebral artery occlusion. Brain magnetic resonance imaging（MRI） examination was performed after the recovery of animal spontaneous breathing. Intraoperative hemodynamic changes,recovery time of spontaneous breathing, additional volume of sumianxin-ketamine mixture,the dosage of propofol,and the incidence of anesthesia related complications were recorded. Results All models survived more than 24 h after the onset of ischemia. No serious complications such as restlessness,respiratory depression,arrhythmia occurred in 16 rhesus monkeys. The hemodynamic indexes,body temperature,and arterial blood gas analysis of 16 rhesus monkeys were in normalrange. The hemodynamic indexes,body temperature,and arterial blood gas analysis showed insignificant difference between the two groups at different intervals（P〈0. 05）. All the 16 monkeys awoke in 30 min after the experiment. The spontaneous breathing recovery time of the two groups showed insignificant difference（P〉0. 05）. Compared with the group P,the dosage of propofol and the volume of sumianxin-ketamine mixture were lower than that in the group PD（P〈0. 05）. Conclusion Two anesthetic procedures are feasible and easy to control the anesthesia depth,which are safe to anesthetize rhesus monkey
作者
马艳辉
吴迪
田肇隆
贾宾
徐娜
陈健
王天龙
Ma Yanhui;Wu Di;Tian Zhaolong;Jia Bin;Xu Na;Chen Jian;Wang Tianlong(Department of Anesthesiology,Xuanwu Hospital,Capital Medical University,Beijing 100053,China;China-America Institute of Neuroscience,Xuanwu Hospital,Capital Medical University,Beijing 100053,China;Department of Neurosurgery,Xuanwu Hospital,Capital Medical University,Beijing 100053,China)
出处
《首都医科大学学报》
CAS
北大核心
2018年第4期552-556,共5页
Journal of Capital Medical University
基金
北京市医院管理局"登峰计划"专项经费资助(DFL20150802)
北京市医管局青苗项目(QML20170802)~~
关键词
恒河猴
大脑中动脉栓塞
全凭静脉麻醉
丙泊酚
右美托咪定
rhesus monkeys
middle cerebral artery occlusion
total intravenous anesthesia
propofol
dexmedetomidine
