摘要
目的探讨哮喘大鼠肺组织细胞线粒体功能及氧化应激水平的变化。方法将24只2~3个月龄雄性Sprague-Dawley大鼠随机分为哮喘模型组和正常对照组,每组12只。哮喘模型组大鼠第1、7天给予100 g·L^(-1)卵清蛋白溶液1 mL腹腔注射致敏;第15天给予30 g·L^(-1)卵清蛋白溶液雾化激发,每日1次,每次30 min,连续15 d,制作哮喘模型;正常对照组大鼠常规饲养。造模成功后水合氯醛麻醉,采集2组大鼠腹主动脉血检测血浆8-异前列腺素(8-iso)、硫代巴比妥物(TBARS)、活性氧(ROS)水平;分离2组大鼠肺组织,透射电镜下观察线粒体超微结构并检测肺组织细胞线粒体膜电位(MMP)、细胞色素C氧化酶(COX)和三磷腺苷(ATP)水平。结果透射电镜下可见,哮喘模型组大鼠肺组织中肺泡Ⅱ型上皮细胞肿胀、脱落;板层体减少、肿胀,线粒体减少、肿胀,嵴结构不清;肺泡间隔增厚,可见嗜酸性粒细胞、巨噬细胞浸润,红细胞淤积。正常对照组大鼠肺组织中肺泡、肺泡Ⅱ型上皮细胞结构正常,肺泡间隔、血管壁无增厚。哮喘模型组大鼠血浆8-iso、TBARS、ROS水平高于正常对照组(P<0.05)。哮喘模型组大鼠肺组织线粒体MMP、COX及ATP水平均低于正常对照组(P<0.05)。结论哮喘大鼠肺组织存在线粒体功能障碍,线粒体功能障碍可能是引起氧化应激的重要因素。
Objective To investigate the changes of mitochondrial function of cells in lung tissue and its effect on oxidative stress in asthmatic rats.Methods Twenty four male Sprague-Dawley rats aged 2-3 months were selected and randomly divided into asthma model group and normal control group,with 12 rats in each group.The rats of asthmatic model group were given 10% ovalbumin solution（1 mL） intraperitoneally sensitized on the first and the seventh day,then 3% ovalbumin solution was given by atomized inhalation on the fifteenth day,one time a day,30 minutes each time for 15 days.The rats in the normal control group was reared routinely.The rats were anesthetized by chloral hydrate after modeling successfully.Abdominal aorta blood of all rats were collected to determine the plasma 8-isoprostane（8-iso）,thiobarbituric acid reaction products（TBARS） and reactive oxygen species（ROS）.The pulmonary tissue was isolated from the rats of the two groups to determine the mitochondrial transmembrane potential（MMP）,activity of cytochrome C oxidase（COX）,adenosine triphosphate（ATP）level and to observe the ultrastructure by transmission electron microscopy.Results Under the transmission electron microscope,the alveolar type Ⅱ epithelial cells in the lung tissue were swollen and shedding,the lamellar body was reduced and swollen,mitochondria decreased and swelling,and the ridge structure was not clear;the alveolar septum was thickened;the eosinophils,macrophages infiltration and red cells deposition were observed in the asthmatic model group.In the normal control group,alveolar structure and alveolar type Ⅱ epithelial cell structure was normal,alveolar septum was not thickened,and no thicken blood vessels wall was observed.The plasma levels of 8-iso,TBARS and ROS in the asthmatic model group was lower than those in the normal control group（P〈0.05）.The MMP,activity of COX,ATP level of lung tissue in the asthmatic model group was lower than those in the normal control group（P〈0.05）.Conclusion Mitoch
作者
刘慧芳
姜敏
马红霞
LIU Hui-fang;JIANG Min;MA Hong-xia(Department of Respiration,Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University,Urumchi 830000,Xinjiang Uygur Autonomous Region,Chin)
出处
《新乡医学院学报》
CAS
2018年第8期662-665,共4页
Journal of Xinxiang Medical University
基金
新疆维吾尔自治区自然科学基金青年项目(编号:2017D01C175)
关键词
哮喘
线粒体功能
氧化应激
大鼠
asthma；mitochondrial function；oxidative stress；rat
