摘要
目的:建立HPLC多波长法测定香砂平胃丸中橙皮苷、厚朴酚、甘草苷、和厚朴酚、苍术素含量的方法。结果:色谱柱为shim-pack VP-ODS C_(18)(4.6 mm×250 mm,5μm);检测波长:237,294,335 nm;流动相选用乙腈(A)-1%醋酸的水溶液(B)15∶85进行梯度洗脱:0~9 min,15%~40%A,9~16 min,40%~80%A,16~25 min,80%~75%A,25~35 min,75%~80%A。流速:1.0 mL·min^(-1);柱温40℃。结果:甘草苷、和厚朴酚、厚朴酚、橙皮苷、苍术素在选择的浓度范围内与峰面积呈现出良好的线性关系。结论:该方法简单,准确,重现性高,灵敏度好,可应用于香砂平胃丸的质量控制。
Objective： HPLC method is established to simultaneous determination of the content for hesperidi liquiritin magnolol honokiol and atisine chloride atractydinby wavelength spectrometry. Methods： The detection wavelength was 237,294,335 nm,The column is Shimadzu VP-ODS（ 4. 6 mm × 250 mm,5 μm）. The flow rate was 1. 0 mL·min^（-1）,and the column temperature was 40 ℃. Mobile phase A： acetonitrile-water; Mobile phase B： 1% acetic acid water（ 15 ∶ 85）,a gradient elution was performed as followed： 0 ~ 9 min,15% ~ 40% A,9 ~ 16 min,40% ~ 80% A,16 ~ 25 min,80% ~ 75% A,25 ~ 35 min,75% ~ 80% A. Results： Hesperidin,liquiritin,magnolol,honokiol,atisinechloride atractydin showed good linearity over the tested ranges. Conclusion： The method for the quality control of Xiangshapingwei Pills is simple,accurate,and applicable
作者
赵艳
侯昕彤
赵磊
ZHAO Yan;HOU Xi-tong;ZHAO Lei(Siping Institutejbr Food and Drug Control,Jill Siping 136000,China;Tianjin university of traditional Chinese medicine,Tianjin,Tianjin 300100,China)
出处
《中国药物评价》
2018年第3期206-208,共3页
Chinese Journal of Drug Evaluation
关键词
香砂平胃丸
甘草苷
和厚朴酚
橙皮苷
厚朴酚
苍术素
高效液相色谱(HPLC)
Xiangshapingwei Pills
Liquiritin
Honokiol
Hesperidin
Magnolol
Atractylodin
High Performance Liquid Chromatog-raphy（ HPLC ）
