摘要
以十六烷基三甲基溴化铵(CTAB)作为超分子模板剂,以柠檬酸(CA)作为螯合剂,利用共模板导向组装法制备得到了两种不同孔径大小的多级孔Cu-MOFs材料,分别为H-Cu-MOF-0.1和H-Cu-MOF-0.2。采用物理吸附法,分别以这两种多级孔Cu-MOFs材料为载体,对氯过氧化物酶(CPO)进行固定,并进行了固定化条件优化和固定化酶性质研究,探讨了固定化酶在染料降解中的应用。固定化条件优化结果表明:在CPO质量浓度2.5μmol·L^(-1),pH 2.75下固定40~50 min,固载量最高可达14.51~21.41 mg·g^(-1),制得固定化酶活性最高。固定化酶性质研究结果表明:固定化酶热稳定性明显高于游离CPO;CPO@H-Cu-MOF-0.2具有较好的重复使用性,在重复使用5次后,仍能保持76.38%的催化活性。染料降解应用研究发现:CPO@H-Cu-MOF-0.2对染料结晶紫的降解效果较好,在结晶紫染料初始浓度低于2.0 mmol·L^(-1)时,10 min内的降解率达到94.63%,催化降解反应的较佳反应温度为30℃,较佳pH为3.0。
Using the cooperative template-directed assembly method, two different ranges of pore sizes of hierarchical porous Cu-MOFs materials, namely H-Cu-MOF-0.1 and H-Cu-MOF-0.2, were prepared by choosing hexadecyl trimethyl ammonium bromide （CTAB） as supramolecular temlplate and citric acid （CA） as chelating agent. Here we report that for the first time, chlorperoxidase （CPO） has been successfully immobilized into the two prepared materials （Cu -MOFs） via physical adsorption, espectively. The optimization of conditions of immobilized and the properties of immobilized enzymes were studied, and the application of immobilized enzymes in the degradation of dye was discussed. The results showed that the concentration of CPO was 2.5 μmol·L-1, the value of pH is 2.75, the immobilized time is 40-50 min, and the biggest loading for the CPO was 14.51-21.41 mg·g^-1; The thermal stability of immobilized CPO was higher than that of the free CPO; CPO@H-Cu-MOF-0.2 shows good repeatability, and it can still remain 76.38% of the catalytic activity after 5 cycles; The effect of CPO@H-Cu-MOF-0.2 degrading crystal violet is ood, the degradation rate was greater than 94.63% in 10 min, when the initial concentration of crystal violet was lower than 2.0 mmol·L^-1, corresponding to the better reaction condition is that the reaction temperature is 30 ℃, the pH value is 3.0.
作者
高霞
张国春
乔成芳
GAO Xia;ZHANG Guo-chun;QIAO Cheng-fang(School of Chemical Engineering and Modern Materials,Shangluo University/Shaanxi Key Laboratory ofComprehensive Utilization of Tailings Resources,Shangluo 726000,Shaanx)
出处
《商洛学院学报》
2018年第4期27-33,共7页
Journal of Shangluo University
基金
商洛市科研计划项目(SK2016-42)
关键词
多级孔MOFs材料
固定化酶
染料降解
hierarchical-pore
MOFs materials
immobilized enzyme
dye degradation
