摘要
目的:制备粒径均一且稳定、载药率和包埋率较高的聚合物脂质纳米球。方法:将HSPC(氢化大豆卵磷脂)与PLGA(聚乳酸-羟基乙酸共聚物)两种材料结合,利用超声复乳法制备聚合物脂质纳米球,采用响应面法优化最佳制备工艺;以HSPC(氢化大豆卵磷脂)与PLGA(聚乳酸-羟基乙酸共聚物)的比例、PVA浓度、超声功率为条件优化制备参数,以粒径为响应值。结果:优化后的最佳工艺参数为:HSPC与PLGA的比例为1:10,PVA浓度为0.66%,超声功率为51.34%(205.36 W)。结论:按最优工艺制备出的聚合物脂质纳米粒的粒径为230 nm左右,多分散系数(PDI)值为0.057,与预测值偏差较小,且粒径分布均一,可作为蛋白及多肽类药物的递送载体。
Objective： In order to prepare stable polymer-lipid nanoparticles with uniform particle size,high drug loading and encapsulation efficiency.Methods： HSPC（Hydrogenated Soybean Phospholipids Mixed Compounds） and PLGA（Poly（lactic-co-glycolic acid）） were combined to prepare the polymer-lipid nanoparticles with ultrasonic double emulsion method.The preparation process was optimized using Response Surface Methodology（RSM）.The ratios of HSPC-PLGA,PVA concentrations and ultrasonic power were employed as three main factors affecting the formation of nanoparticles in the preparation process.The particle size was chose as the response value.Results： The optimal conditions were obtained as follows： the ratio of HSPC-PLGA was 1：10,the concentration of PVA was 0.66%,and the ultrasonic power was 51.34%（205.36 W） respectively.Conclusion： The size of nanoparticles prepared under the optimal conditions was about 230 nm with narrow size distributions,and the PDI value was 0.057,which was closer to the predicted value.The established polymer-lipid nanoparticles could be used as protein and polypeptide drugs in delivery system.
作者
孟丹
邱越佳
杨婷媛
王连艳
王硕
朱艳华
马光辉
MENG Dan;QIU Yue-jia;YANG Ting-yuan;WANG Lian-yar;WANG Shuo;ZHU Yan-hua;MA Guang-hui(College of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin,Heilongjiang,150040,China;National Key Lab.Biochem.Eng.,Institute of Process Engineering,Chinese Academy of Sciences,Beijing,100190,China)
出处
《现代生物医学进展》
CAS
2018年第10期1887-1891,共5页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(21476243)
关键词
脂质纳米球
超声复乳法
响应面法
蛋白类药物
Polymer-lipid nanopartieles
Ultrasonic double emulsion method
Response surface methodology
Protein drugs
