摘要
目的 探讨孕期高脂摄入对雄性子代幼年期及成年期血脂水平、肝脏脂质沉积的影响,以及孕期高脂暴露对雄性子代肝脏固醇调节级联通路重要因子,即固醇调节元件结合蛋白-l（sterol regulatory element binding protein-1,SREBP-1）和SREBPs裂解激活蛋白（SREBP cleavage acting protein,SCAP）表达的影响. 方法 雌性Wistar大鼠受孕成功后,随机分为高脂组和对照组,每组20只.高脂组孕鼠高脂饲料喂养至分娩,后改为普通饲料喂养;对照组孕鼠一直普通饲料喂养.孕鼠分娩后,从高脂组和对照组中随机选择适于胎龄雄性仔鼠作为实验对象;比较2组雄性仔鼠7周龄、24周龄时体格发育、血脂水平及肝脏脂质沉积情况;免疫组织化学技术测定SCAP及SREBP-1蛋白表达水平.采用f检验、x2检验对数据进行统计学分析. 结果 高脂组仔鼠的平均出生体重高于对照组[（6.95±0.25）与（6.79±0.78）g,t=2.088,P=0.038].高脂组仔鼠大于胎龄、适于胎龄、小于胎龄分别为18.6％ （21/113）、77.9％（88/113）、3.5％ （4/113）,对照组分别为7.6％ （10/132）、87.9％（116/132）、4.5％ （6/132）,差异有统计学意义（x2=13.500,P=0.001）.7周龄时,高脂组雄性仔鼠体重高于对照组[（68.78±7.55）与（66.61±3.92）g,t=2.303,P=0.023].24周龄时,高脂组雄性仔鼠的体重、身长、腹围均高于对照组[（251.74±24.04）与（216.24±33.42）g,（22.60±0.79）与（21.59±1.34） cm,（17.93±0.59）与（16.83±0.88）cm,t值分别为2.220、2.379及2.927,P值均＜0.05].7周龄时,高脂组雄性仔鼠的总胆固醇水平高于对照组[（1.94±0.62）与（1.23±0.77）mmol/L,t=2.379,P＜0.05];24周龄时,高脂组雄性仔鼠的肝脏指数、总胆固醇、甘油三酯、低密度脂蛋白-胆固醇、极低密度脂蛋白-胆固醇均高于对照组,但高密度脂蛋白-胆固醇低于对照组[（2.75±0.31）％与（2.46±0.39）％,（1.98±0.14）与（1.45±0.27）mmol/L,（2.96�
Objective To analyze the influences of maternal high-fat diet on male rat offspring＇s blood lipid level and hepatic lipid deposition as well as on the expression of two key factors,sterol regulatory element binding protein-1 （SREBP-1） and SREBP cleavage acting protein （SCAP）,involved in hepatic cholesterol regulatory cascade during childhood and adulthood.Methods Pregnant Wistar rats were randomly divided into two groups：high fat diet （HF） group and normal intake control （NC） group （both n=20）.Rats in the HF group were fed with high-fat diet till delivery before changing to a normal-fat diet,while the NC group was given the normal-fat diet all through the study.Male offspring born appropriate for gestational age （AGA） in different feeding groups were randomly selected as experimental subjects.Physical development,serum lipid levels and hepatic lipid deposition at the age of 7 and 24 weeks were compared between offspring of the two groups.Expression of SCAP and SREBP-1 at protein level was detected with immunohistochemistry.T test or Chi-square test was used for statistical analysis.Results The average birth weight of HF group was higher than that of NC group [（6.95± 0.25） vs （6.79 ±0.78） g,t=2.088,P=0.038].Large for gestational age （LGA）,AGA and small for gestational age （SGA） offspring accounted for 18.6% （21/113）,77.9% （88/113）and 3.5% （4/113） in HF group and 7.6% （10/132）,87.9% （116/132） and 4.5% （6/132） in NC group （x2=13.500,P=0.001）.At the age of 7 weeks,the offspring＇s body weight in HF group was higher than that in NC group [（68.78±7.55） vs （66.61 ±3.92） g,t=2.303,P=0.023].At the age of 24 weeks,the body weight,body length and abdominal circumference of adult offspring in HF group were significantly higher than those in NC group [（251.74±24.04） vs （216.24±33.42） g,（22.60±0.79） vs （21.59± 1.34） cm,（17.93±0.59） vs （16.83±0.88） cm;t=2.220,2.379,2.927,all P〈0.05].At the age of 7 weeks,offsprin
作者
华绍芳
苏莉军
许丽萍
Hua Shaofang;Su Lijun;Xu Liping(Department of Obstetrics,the Second Hospital of Tianjin Medical University,Tianjin 300211,China;Department of Pathology,the Second Hospital of Tianjin Medical University,Tianjin 300211,China;Department of Obstetrics,Henan Provincial People＇s Hospital,Zhengzhou 450003,China)
出处
《中华围产医学杂志》
CAS
CSCD
北大核心
2018年第8期558-564,共7页
Chinese Journal of Perinatal Medicine
基金
天津市卫生局科技基金（2012KZ084）
