摘要
分别采用SBR反应器和MBR反应器驯化培养亚硝化污泥和厌氧氨氧化(anaerobic ammonia oxidation,ANAMMOX)污泥,并通过微生物包埋技术将两类污泥分别包埋,构建亚硝化-厌氧氨氧化(partial nitrification-ANAMMOX,PN/A)双菌层系统.短期实验证明该系统中亚硝化菌(ammonia oxidizing bacteria,AOB)和ANAMMOX菌在不同阶段分别起主导作用,维持系统的酸碱平衡,并实现NH+4-N的高效去除(98.8%).长期实验表明,在溶解氧受限时,PN/A双菌层系统能够有效提高系统对溶解氧的利用效率,并增强系统的稳定性和脱氮效能.在溶解氧为1.0 mg·L^(-1),进水NH+4-N质量浓度分别为200 mg·L^(-1)和400 mg·L^(-1)时,对照组脱氮效率仅为58.1%和61.4%,而PN/A双菌层系统脱氮效率均稳定在80%左右;溶解氧为3.0mg·L^(-1),进水NH+4-N质量浓度为400 mg·L^(-1)时,PN/A双菌层系统总氮去除率达87.9%,总氮积累负荷(NLR)为0.4kg·(m3·d)-1,总氮去除负荷(NRR)为12.8 mg·(g·h)-1.
Nitrification and anaerobic ammonia oxidation （ANAMMOX） sludge was acclimated via SBR and MBR reactors respectively, both of which were embedded with microbial immobilization technology and spliced together to construct a PN/A double-bacteria-layer system. A short-term experiment proved that ammonia-oxidizing bacteria （AOB） and ANAMMOX bacteria in the system played dominant roles separately in different stages, which maintained the acid-base balance and achieved efficient removal of NH4＋ -N （98.8%）. A long- term experiment showed that the PN/A double-bacteria-laye~ system improved the utilization of dissolved oxygen （DO） and enhanced the stability and nitrogen removal efficiency, with limited dissolved oxygen. The nitrogen removal efficiency of the PN/A double-bacteria- layer system remained stable at -80% when the influent NH4＋ -N was 200mg·L-1 or 400 mg·L-1 in the presence of 1.0 mg·L-1 DO, whereas those of the control group were only 58.1% and 61.4% , respectively. When influent NH4＋ -N and DO were 400 mg·L-1 and 3.0mg·L-1, respectively, the PN/A double-bacteria-layer system achieved a nitrogen removal efficiency, nitrogen loading rate （NLR） , and nitrogen removal rate （NRR） of 87.9% , 0. 4 kg· （ m3· d） - 1 , and 12. 8 mg·（ g· h） - 1 , respectively.
作者
许静怡
杜俊
杨一烽
吕锋
夏四清
XU Jing-yi;DU Jun;YANG Yi-feng;LU Feng;XIA Si-qing(College of Environmental Science and Engineering,Tongji University,Shanghai 200092,Chin)
出处
《环境科学》
EI
CAS
CSCD
北大核心
2018年第8期3767-3774,共8页
Environmental Science
基金
国家重点研发计划项目(2017YFC0403400)
关键词
亚硝化
厌氧氨氧化
微生物包埋
溶解氧
脱氮效能
partial nitrification
anaerobic ammonia oxidation （ANAMMOX）
microorganism immobilization technology
dissolved oxygen （DO）
nitrogen removal efficiency
