摘要
探讨鞘氨醇-1-磷酸（sphingosine-1-phosphate,S1P）对少突胶质前体细胞（oligodendrocyte precursor cells,OPCs）的影响及其作用机制。使用P3SD乳鼠构建OPCs原代培养体系,运用倒置相差显微镜、CCK-8、流式细胞术（FCM）、Ed U及Western blotting检测细胞增殖情况和蛋白表达量。结果证实S1P能促进OPCs增殖,并且随着S1P浓度升高呈上升趋势,在10滋mol/L时细胞增殖能力最佳;其增殖机制是通过与S1PR-3结合,激活PI3K/p-ERK信号通路,使S期细胞比例上调。OPCs可增殖、迁移和分化到损伤部位进行抗髓鞘修复,因此关于OPCs增殖的深入研究对于脱髓鞘疾病的临床治疗具有重大意义。
In order to investigated the effects and mechanisms of sphingosine-1-phosphate（S1 P） on oligodendrocyte precursor cells（OPCs）.Primary culture systems of OPCs were established by neonatal Sprague-Dawley rats（P〈0-P3）.Light microscopy,CCK-8,FCM,Ed U and Western blotting were used to investigate the proliferation of OPCs and the protein expression.The results showed that S1 P can promote OPCs proliferation and the OPCs proliferation is on the rise with elevated S1 P concentration.The mechanisms of S1 P promoted OPCs proliferation was to combined with S1 PR-3 and to activated PI3 K/p-ERK pathway which can regulate cell cycle by promoting G1 phase into S phase.OPCs will proliferation,migration and differentiation to the injured part for repairing,so in-depth study about OPCs proliferation plays an important role for clinical treatment of demyelinating diseases.
作者
高晓燕
姜露
徐丹
张文锦
唐勇
彭彦
Gao Xiaoyan;Jiang Lu;Xu Dan;Zhang Wenjin;Tang Yong;Peng Yan(Chongqing Medical University,Chongqing,400016;The First Affiliated Hospital of Chongqing Medical University,Chongqing,400016)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2018年第8期3635-3640,共6页
Genomics and Applied Biology
基金
国家自然科学基金(81671259
81470057
81501101)
重庆市“百名学术学科领军人才”培养计划
重庆市渝中区科技计划项目《星形胶质细胞通过CX47激活SIPR3介导少胶前体细胞增殖机制研究》共同资助
