摘要
目的探讨甘草次酸(GA)对于糖尿病肾病(DN)的治疗效果。方法利用CS7 BL/6背景的小鼠和db/db小鼠分别构建1型和2型DN的动物模型。将C57 BL/6小鼠分别分为正常对照组(Con组)、甘草次酸处理的对照组(Con+GA组)、DN组和甘草次酸处理的DN组(DN+GA组),db/db小鼠分为对照组、DN组和治疗组。然后分别观察GA处理对于小鼠血糖、体质量、尿肌酐、尿蛋白/肌酐的比值、细胞外基质的沉积以及细胞凋亡的影响。结果和Con组的血糖相比,DN组血糖水平和尿蛋白/肌酐的比值显著升高,差异有统计学意义(P<0.01);细胞外基质的沉积和细胞凋亡增加;尿肌酐的排泄显著减少,差异有统计学意义(P<0.01)。和DN组相比,DN+GA组小鼠尿肌酐的水平以及尿蛋白/肌酐的比值有显著性差异(P<0.01);细胞外基质的沉积明显缓解;细胞凋亡水平下降。1型DN动物模型中,Con组和DN组小鼠体质量差异有显著性(P<0.05),同时DN组和DN+GA组小鼠体质量差异有显著性(P<0.01)。结论 GA对于1型以及2型DN动物模型中肾脏的病变均能够发挥保护作用,从而延缓肾脏疾病的进展。
Objective To study the effect of glycyrrhetinicacid on the diabetic nephropathy（DN） animal model. Methods In order to estimate the effect of glycyrrhetinicacid treatment on the blood glucose,body weight,urine creatinine,the ratio of urinary protein/creatinine,the sedimentation of extracellular matrix and the apoptosis of kidney cells,we built the animal models of type one and type two DN. Type one mouse model of DN included control group,control group plus glycyrrhetinicacid, DN group and DN group plus glycyrrhetinicacid. Type two DN model included control group,DN group and treatment group. Results Compared with the control group,the level of blood glucose and the ratio of urinary protein/creatinine were significantly increased in DN group and the difference was statistically significant（P〈0. 0 1）. The deposition of extracellular matrix and the apoptosis of kidney cells increased,whereas the level of urina creatinine decreased in DN group as compared with control group and the difference was statistically significant（P〈0. 0 1）. As compared with DN group,the level of urine creatinine and the ratio of urinary protein/creatinine in DN plus glycyrrhetinicacid group were significantly different（P〈0. 0 1）,the sedimentation of extracellular matrix obviously relieved and the apoptosis of kidney cells decreased. In type one DN model,there was significant difference in body mass between Control group and DN group（P〈0. 0 5） and at the same time,there was significant difference in body mass between DN group and DN plus glycyrrhetinicacid group（P〈0. 0 1）. Conclusions The treatment of GA could protect against the renal lesions of type one and type two DN animal model,thereby delaying the progress of the kidney diseases.
作者
李小琴
沈莹
石珺
LI Xiao-qin;SHEN Yin;SHI Jun(Department of Nephropathy,Liyuan hospital,Tongji Medical College,Huazhong U niversity of Science and Technology,Wuhan 430000,Chin)
出处
《临床肾脏病杂志》
2018年第7期434-439,共6页
Journal Of Clinical Nephrology
关键词
甘草次酸
糖尿病肾病
凋亡
ERK信号通路
Glycyrrhetinicacid
Diabetic nephropathy
Apoptosis
ERK signaling pathway
