摘要
[目的]研究肉种鸡共轭亚油酸(CLA)饲粮中添加大豆油对其繁殖性能的影响,最终确定使CLA种蛋孵化率较好恢复的大豆油添加量。[方法]选36周龄健康肉种母鸡600只,种公鸡72只,分为6个组,每组设4个重复,每重复28只,其中母鸡25只,公鸡3只。第1组为对照组,饲喂普通饲粮,大豆添加量为0.5%,2~6组为试验组,饲喂分别补充0%、2%、4%、6%、8%大豆油的CLA饲粮(含0.5%CLA油),1~6组营养水平一致。测定种蛋的孵化率、蛋品质、蛋黄脂肪酸和抗氧化指标。[结果]2组受精蛋孵化能力基本丧失,种鸡CLA饲粮中补充不同比例的大豆油,受精蛋孵化率会有不同程度的改善,添加4%~5%的大豆油时,受精蛋孵化率能够较好恢复,大豆油的添加量为8%时,受精蛋孵化率并没有继续改善。各组的蛋品质没有显著差异(P>0.05)。种蛋的脂肪酸构成发生显著改变,2组的饱和脂肪酸含量显著大于1组(P<0.05),单不饱和脂肪酸含量显著低于1组(P<0.05),多不饱和脂肪酸含量与1组差异不显著(P>0.05),而C20∶4n6含量显著低于1组,CLA-C_9t_(11)、CLA-t_(10)c_(12)含量显著大于1组(P<0.05);3、4、5、6组(添加0.5%CLA油+不同比例的大豆油)的上述饱和脂肪酸含量低于2组,且随着大豆油添加比例的增加而逐渐降低,单不饱和脂肪含量变化规律不明显,多不饱和脂肪酸的含量逐渐增加。各组种蛋的抗氧化能力有显著差异,1组和4组总抗氧化能力显著高于其他组(P<0.05),相应,种蛋中的过氧化产物丙二醛(MDA)含量显著低于其他各组(P<0.05)。[结论]该研究中,种蛋的孵化率受种蛋中脂肪酸构成以及种蛋抗氧化能力的影响,添加适当的大豆油能调整种蛋脂肪酸的构成,从而使由CLA所致的种蛋孵化能力降低得到较好的恢复。
[Objective]To determine the effects of conjugated linoleic acid diets supplemented with different proportions of soybean oil on reproductive performance of chicken breeders,and ascertain the minimum level of dietary plant oil supplementation that results in full recovery from loss of hatchability induced by conjugated linoleic acid.[Method]600 hens and 72 cocks of 36 weeks were assigned to groups of 6 and were fed a diet containing either no CLA plus 0. 5% soybean oil（ control） or 0. 5% CLA plus 0%,2%,4%,6% or 8% soybean oil. The hatching rate of eggs,egg quality,yolk fatty acid and antioxidant index were measured.[Result]Supplementation with CLA（ CLA plus 0% soybean oil） resulted in almost loss of hatchability of fertile eggs. Hatchability was progressively improved by increasing doses of soybean oil,and better recovery of hatchability compared with the control levels was achieved at 4% ~ 5% soybean oil. There was no further improvement in hatchability when 8%soybean oil was added to the CLA-supplemented diet. There was no significant difference in egg quality in each group（ P0. 05）. The fatty acid composition of eggs was significantly changed. The contents of saturated fatty acids in the group 2 were significantly higher than those in the group1（ P0. 05）,the contents of monounsaturated fatty acids were significantly lower than those in the group 1（ P0. 05）,and the contents of polyunsaturated fatty acids in the group 1 were not significantly different from those in the group 1（ P0. 05）. But the content of C20 ∶4 n6 was significantly lower than the group 1,the contents of CLA-C9t（11） and CLA-t（10）c（12） were significantly greater than that of the group 1（ P0. 05）; the saturated fatty acid contents of group 3,4,5,6 were lower than the group 2,and with the increasing proportion of soybean oil decreased,single variation of unsaturated fat content was not obvious,the content of polyunsaturated fatty acids increased gradually. The antioxidant capacity of eggs in each group was
作者
刘雪兰
张燕
伏春燕
魏祥法
阎佩佩
石天虹
董以雷
刘瑞亭
LIU Xue-lan;ZHANG Yan;FU Chun-yan(Poultry Institute,Shandong Academy of Agricultural Sciences,Jinan,Shandong 25002)
出处
《安徽农业科学》
CAS
2018年第19期96-99,共4页
Journal of Anhui Agricultural Sciences
基金
山东省自然科学基金项目(ZR2015YL062)
关键词
共轭亚油酸
大豆油
孵化率
脂肪酸
种鸡
Conjugated linoleic acid
Soybean oil
Hatching rate
Fatty acid
Chicken breeders
