摘要
目的:研究匍匐大戟醇的提物在肿瘤的对抗作用以及在血管新生的抑制作用方法取正常的细胞和肿瘤细胞,用药之后通过MTT的方法来对IC50进行检测。原代对大鼠胸主动脉内皮细胞(RAECs)进行培养,进行细胞的分析实验,用的是体外i CELLigence的方法,此外,还进行了细胞迁移法和细胞管组实验的实验方法,研究了匍匐大戟提取物对RAECs的增量、迁移和城市管理的影响,同时对p-Akt,Akt,p-e NOS,e NOS,TGF-β1,Smad3等血管新生相关信号通路蛋白的表达情况进行检测,检测的方法是Western blot技术。通过对人肝癌裸鼠移植瘤模型的建立,予以匍匐大戟醇提物,裸鼠体重、肿瘤大小、肿瘤等质量检测,同时对每组裸鼠血清血管内皮生长因子(VEGF)和血小板源性生长因子(PDGF-BB)水平进行检测,检测的方法是LISA技术方法,同时还要对移植瘤组织的形态进行观察,移植瘤组织是进行过HE染色的。结果:匍匐大戟的提纯物对小鼠的最大的耐受量MTD是94.29/(g·kg)。对于大鼠成肌细胞(L6细胞株),匍匐大戟醇提物没有明显的抑制作用,而对于对鼠肾上腺嗜铬细胞瘤细胞株PC12、人肝癌细胞Hep G-2、大、人非小细胞肺癌细胞株A549、人宫颈癌细胞细胞株Hela来讲,匍匐大戟有明显的抑制作用,此外匍匐大戟醇提物能够增加RAECs的值、迁移和试管的形成,也会有显著的抑制作用。结论:匍匐大戟醇提物能够对肿瘤的生长和血管的生成具有很显著的抑制作用,这种情况也是匍匐大戟发挥抗肿瘤作用的有效机制之一。
Objective ： To explore the antagonism of ethanol extract from Euphorbia stolondi in tumor and its inhibitory effect on an- giogenesis. Methods：Normal cells and tumor cells were cultured, and IC50 was detected by MTT after medication. The primary rat thoracic aorta endothelial cells （RAECs） were cultured and analyzed by iCELLigenee method. The effects of Euphorbia extract on the increment, nfigration and vaseularization of RAECs were studied by cell nfigration method and cell tube group. Western blot technology was used to detect the expression of p-Akt, Akt, p-eNOS, eNOS, TGF-β1, Smad3 and other proteins related with angio-genesis signaling pathways. When the model of human liver cancer established with nude nfiee carried transplanted tumor after in-tervened by prostrate Euphorbia alcohol extract, the body weight, tumor size and tumor quality of nude mice were detected, and the levels of vascular endothelial growth factor （VEGF） and platelet derived growth factor （PDGF-BB） in the serum of nude mice were detected by LISA technique. At the same time, the morphology of transplanted tumor ,which is stainined by HE, is ob-served. Results ：The maximum MTD tolerance of the extract from Euphorbia stolon was 94.29g · kg-1. For the rat myoblasts （L6 cell lines）, the prostrate alcohol extract has no obvious inhibitory effect, while the prostrate Euphorbia has convincing inhibitory effect on the rat adrenal pheoehromoeytoma cell line PC12, human hepatoma cell HepG-2, human non-small cell lung cancer cell line A549 and human cervical cancer cell Hela. In addition, ethanol extract of Euphorbia stolon can increase the RAECs value, migration and vaseularization, and also have a significant inhibitory effect. Conclusion：The prostrate alcohol extract can signifi-cantly inhibit the growth of tumor and the formation of blood vessels, which may be one of the effective mechanisms of the antitumor effect of the Euphorbia prostrate.
作者
李国强
李岩
Li Guojiang;Li Yan(Cancer surgery in Xinyang Central Hospital of Henan,Xinyang 464000,China;Xinwen Mining Central Hospital of Refco Group Ltd,Xintai 271233,China)
出处
《世界中医药》
CAS
2018年第5期1266-1269,共4页
World Chinese Medicine
关键词
匍匐大戟
肿瘤
血管新生
Euphorbia prostrate
Tumor
Angiogenesis
