摘要
目的研究重组人可溶性血栓调节蛋白(ART-123)对小鼠脑缺血/再灌注损伤的影响。方法 BALB/c小鼠行4 h大脑中动脉闭塞(MCAO),再灌注时静脉注射Vehicle或ART-123(1或5 mg/kg)。再灌注24 h后评估小鼠脑梗死体积、运动协调、血浆高迁移率族蛋白(HMGB1)水平及出血量。结果 ART-123可减少MCAO小鼠脑梗死体积,并呈剂量依赖性(F=4.843,P=0.038)。与Vehicle组比较,ART-123(5 mg/kg)可改善旋转试验中MCAO小鼠运动协调(P=0.028);降低血浆HMGB1水平(P=0.000)。此外,Vehicle组和ART-123组小鼠出血量差异无统计学意义(P>0.05)。结论 ART-123可能通过抑制HMGB1改善小鼠脑缺血/再灌注损伤。
Objective To investigated the effects of recombinant human soluble thrombomodulin alpha（ART-123） on cerebral ischemic/reperfusion injury in mouse model. Methods BALB/c mice were subjected to 4 h-middle cerebral artery suture occlusion（MCAO） and treated at reperfusion with Vehicle or ART-123（1 or 5 mg/kg, i.v） after 4 h MCAO. Infarct volume, motor coordination, plasma high-mobility group box（HMGB1） level and hemorrhage volume were evaluated 24 h after 4 h MCAO. Results The infarct volume was reduced by ART-123 in mice subjected to 4 h MCAO in a dose-dependent manner（F = 4.843, P = 0.038）. Moreover, ART-123（5 mg/kg） improved motor coordination determined by the rotarod test（P = 0.028）, and decreased plasma HMGB1 level compared with Vehicle-treated controls（P = 0.000）. In addition, there was no difference in hemorrhage volume between Vehicletreated control group and the ART-123 treatment group. Conclusions ART-123 may improve cerebral ischemia/reperfusion injury in mice by inhibiting HMGB1.
作者
黄山鉴
韦贵宁
姚人银
邓小玲
黄祖贵
Shan-jian Huang;Gui-ning Wei;Ren-ying Yao;Xiao-ling Deng;Zu-gui Huang(Department of Pharmacy,Qinzhou First People＇s Hospital,the Tenth Afliated Hospital of Guangxi Medical University,Qinzhou,Guangxi 535000,China;Guangxi Traditional chinese Medicine and Natural Medicine Engineering Technology Research Center,Nanning,Guangxi 530022,China)
出处
《中国现代医学杂志》
CAS
2018年第18期29-33,共5页
China Journal of Modern Medicine
