摘要
探讨结核分枝杆菌(Mycobacterium tuberculosis,Mtb)感染Ⅱ型肺泡上皮细胞(Alveolar epithelial typeⅡcells,AECⅡ)的免疫应答过程中,Hedgehog与NF-κB信号通路及炎症细胞因子的表达变化规律。利用结核分枝杆菌BCG(Bacillus Calmette-Guérin,BCG,卡介苗,弱毒株)和H37Rv(国际标准强毒株)株分别感染人AECⅡ细胞株A549,利用q PCR和Western blot检测Hedgehog信号通路以及NF-κB信号通路相关因子和炎症细胞因子的表达变化。结果表明,H37Rv和BCG感染A549细胞后,Hedgehog信号通路中Shh、Ptch和Gli1与NF-κB信号通路中p-NF-κB m RNA的表达水平升高,同时,伴随着炎症细胞因子IL-8和TNF-αm RNA的表达水平也升高。并且,H37Rv感染A549细胞引起信号分子Shh、Ptch、Gli1和NF-κB蛋白的表达水平高于BCG。Mtb感染AECⅡ细胞都能激活Hedgehog信号通路和NF-κB信号通路并促进细胞的炎症因子分泌,强毒株感染对信号通路的活化强于弱毒株。
In order to investigate the roles of expression of Hedgehog and NF-κB signaling pathway signal molecules and inflammatory cytokines in the alveolar epithelial typeⅡ cells（AECⅡ） infected with Mycobacterium tuberculosis（Mtb）,the human AEC Ⅱ cell line A549 was infected with Bacillus Calmette-Guérin（BCG,attenuated strain） and H37 Rv（international standard virulent strain） respectively,and the signal molecules of Hedgehog and NF-κB signaling pathway and inflammatory cytokines in A549 cells of immune response to Mtb was detected by q PCR,and Western blot. The results showed that, the expression of Shh,Ptch and Gli1 in Hedgehog signaling pathway and the phosphorylation of NF-κB in NF-κB signaling pathway was increased in A549 cells when infected with H37 Rv and BCG,and at the same time,the expression of IL-8 and TNF-α was also upregulated. And the protein expression of Shh,Ptch,Gli1 and NF-κB in A549 cells infected with H37 Rv was higher than that infected with BCG. The Hedgehog and NF-κB signaling pathway could be activated, and the expression of inflammatory cytokines could be promoted in AECⅡ cells when infected with Mtb. The activation level of the both signaling pathway infected with Mtb virulent strain was higher than that of infected with BCG strain.
作者
杜军
任奇杰
王媛
王娟
李勇
DU Jun;REN Qi-jie;WANG Yuan;WANG Juan;LI Yong(Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China;School of Life Science,Ningxia University,Yinchuan 750012,China)
出处
《湖北农业科学》
2018年第11期70-74,90,共6页
Hubei Agricultural Sciences
基金
国家自然科学基金项目(31560694)
宁夏高校科学研究优秀青年基金项目(NGY2015052)
宁夏自然科学基金项目(NZ15017)
