摘要
为研究转EPSPS基因抗除草剂玉米CC-2的种植对土壤跳虫群落的影响,探讨土壤跳虫对环境因子变化的响应,采用土漏斗法收集2014年5月至10月的转基因抗除草剂玉米CC-2及其非转基因对照玉米郑58田间土壤跳虫样品,分析其群落组成、形态特征值和群落多样性指数。在此基础上,采用冗余分析(Redundancy analysis,RDA)探讨了土壤跳虫群落与环境因子之间的关系。结果表明,转基因抗除草剂玉米CC-2与其非转基因对照玉米郑58田间土壤跳虫群落组成基本一致。重复测量方差分析结果表明:两种玉米材料田间土壤跳虫群落形态特征值(眼睛数量、体长、体色、弹器发达程度、触角长度)均无显著差异;两种玉米材料田间土壤跳虫群落多样性指数(物种丰富度、个体数、Simpson优势度指数、Shannon-Wiener多样性指数和Pielou均匀性指数)均无显著差异。冗余分析表明,玉米根部生物量和样地含水量对土壤跳虫群落产生了显著影响,而玉米材料则未对其产生显著影响。初步结论为,转基因抗除草剂玉米CC-2的种植对土壤跳虫群落无不利影响。
The objective of the present study is to investigate the effects of cultivating transgenic herbicide-resistant corn CC-2, with the EPSPS gene, soil Collembola community. From May to October 2014, soil collembolans were extracted from both transgenic and control（Zheng 58）corn, using the Macfadyen method, and the community composition, morphology, and diversity of the samples were measured.Redundancy analysis was also used to investigate relationships between Collembola community and environmental factors of the soil. The composition and diversity（species richness, density, Simpson index, Shannon-Wiener diversity index, and Pieou index） of the Collembolan communities from the transgenic and control corn were similar, whereas the morphological characteristics（oceli number, body length, body pigmentation, furca development, and antenna length）of the communities were different, although not significantly（repeated measures ANOVA）. Redundancy analysis indicated that corn root biomass and water content, but not corn variety, significantly affected Collembola community. The preliminary conclusion of the present study is that the cultivation of transgenic herbicide-resistant corn CC-2 has no adverse effect on soil Collembola community.
作者
范春苗
王柏凤
周蕾
尹俊琦
宋新元
FAN Chun-miao;WANG Bai-feng;ZHOU Lei;YIN Jun-qi;SONG Xin-yuan(College of Life Science,Jilin Agricultural University,Changchun 130118,China;Institute of Agricultural Biotechnology,Jilin Academyof Agricultural Sciences,Changchun 130033,China;The Key Laboratory of Jilin Agricultural Biological Technology,Changchun 130033,China;College of Life Science and Technology,Harbin Normal University,Harbin 150025,China)
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2018年第6期1203-1210,共8页
Journal of Agro-Environment Science
基金
转基因生物新品种培育重大专项(2016ZX08011-003)
国家自然科学基金项目(31500345)~~
关键词
转基因抗除草剂玉米
跳虫
冗余分析
形态特征值
环境因子
transgenic herbicide-resistant corn
Collembola
redundancy analysis
morphological characteristic value
environmental factor
