摘要
为了对典型荧蒽降解菌(青霉素菌DTQ-HK1)固定化方法进行优选及其荧蒽的降解性能进行探究,比较菌种固定化后的耗氧速率及对荧蒽的短期降解效果,优选出最佳固定化方法,并对其进行液相和底泥中荧蒽长期降解实验.结果显示:粒径为10目玉米芯吸附固定化菌种后的耗氧速率最高为102 mg/(g·d),2%PVA+3%SA的载体包埋固定化后菌种的耗氧速率最高为82 mg/(g·d);对2种固定化方法中的最佳载体进行固定化菌对荧蒽的降解实验,14 d后吸附和包埋固定化菌对荧蒽的降解率分别为28.28%、23.49%,说明吸附固定化菌相对来说对荧蒽的降解和微生物活性更有利.菌种经过最佳固定化方法处理后对液相中荧蒽42 d连续降解实验可知,吸附固定化菌对荧蒽的降解率达到62.45%,比悬浮菌提高了10.23%;而吸附固定化菌对菖蒲根际底泥中荧蒽经过90 d降解实验,降解率达86.14%,比悬浮菌提高了5.56%.
In order to optimize immobilization methods of typical fluoranthene-degrading strain（Penicillium purpurogenum strain DTQ-HK1） and study the fluoranthene-degrading characteristic, the oxygen consumption rate of immobilized strain and short-term degradation of fluoranthene were compared to optimize the immobilization methods,and then the long-term degradation rates of fluoranthene in liquid and river sediment were investigated. The results showed that the oxygen consumption rate of corncob with particle size of 10 mesh immobilized by adsorption was 102 mg/（g·d）, and that of 2% PVA ＋ 3% SA immobilized by embedment was 82 mg/（g·d）. And then 14 day degradation rates of fluoranthene in liquid for the two carriers were 28.28% and 23.49% respectively（for adsorption and embedment carrier）, the result showed that the adsorption immobilization was superior to embedment immobilization about the oxygen consumption rate and degradation rate of fluoranthene. The degradation rate of fluoranthene by adsorption immobilized strain in liquid was 62.45% after 42 days, which was increased by10.23% compared with the free strain, and the degradation rate was 86.14% in river sediment after 90 days,which was increased by 5.56% compared with the free strain.
作者
张朝晖
左宇环
李腾飞
吴降麟
陈萌萌
岳喜明
ZHANG Zhao-hui1,2,ZUO Yu-huan1,LI Teng-fei1,WU Jiang-lin1,CHEN Meng-meng1,YUE Xi-ming1(1. School of Environmental and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300387, China, 2. State Key Laboratory of Separation Membranes and Membrane Processes, Tianjin Polytechnic University, Tianjin 300387, Chin)
出处
《天津工业大学学报》
CAS
北大核心
2018年第3期36-42,共7页
Journal of Tiangong University
基金
国家自然科学基金资助项目(51678408
51508385)
国家重点研发计划项目(2016YFC0400506)
天津市自然科学基金项目(14JCQNJC09000)
关键词
荧蒽降解菌
微生物固定化
河道底泥
生物降解
PAHS
降解性能
fluoranthene degrading strain
microbial immobilization
river sediment
biodegradation
PAHs
degradation characteristic
