摘要
目的:研究miR-940对胃癌细胞增殖的影响,探讨miR-940和Cbl-b信号转导通路在此过程中的作用。方法:采用Real-time PCR法检测胃癌细胞中miR-940的表达,MTT法检测胃癌细胞的增殖能力,双荧光素酶报告基因检测系统检测miR-940与Cbl-b的结合,Western blotting实验观察蛋白的表达。结果:MTT法显示miR-940可促进胃癌细胞MGC803的增殖,BLAST对比分析结果显示Cbl-b与miR-940存在结合位点。双荧光素酶报告基因检测系统证实Cbl-b是miR-940的靶基因。Western blotting结果显示过表达miR-940后,Cbl-b的表达明显下调。Cbl-b抑制胃癌细胞MGC803的增殖。miR-940通过负向调节Cbl-b的表达促进胃癌细胞的增殖。结论:miR-940通过抑制Cbl-b的表达,促进胃癌细胞MGC803的增殖。
Objective ：To investigate the effect of the proliferation of miR - 940 on gastric cancer cell lines, and to explore the role of miR -940 and Cbl -b signal pathways in this process. Methods：Real -time PCR detected the ex- pression of miR -940 on gastric cancer cell lines. MTT detected the proliferation of gastric cancer cells. Dual lucifer- ase reporter assay detected the bingding ability- between miR - 940 and Cbl - b. The expression of the protein was tes- tified by Western blotting analysis. Results ： MTT showed that miR - 940 promoted the proliferation of MCC803. A pu- tative seed sequence of miR - 940 was found in the 3＂ UTR of the Cbl - b transcript. Dual luciferase reporter assay showed that Cbl - b was the target gene of miR - 940. Western blotting showed that minfic miR - 940 inhibited the ex- pression of Cbl - b. Cbl - b suppressed the proliferation of MCC803. miR - 940 promoted the proliferation of MCC803 through inhibiting the expression of Cbl -b. Conclusion ：miR -940 inhibited the proliferation of M6C803 through in- hibiting the expression of Cbl - b.
作者
范一博
车晓芳
赵欢
侯科佐
张敏
曲秀娟
胡雪君
刘云鹏
Fan Yibo;C he Xiaofang;Zhao Huan;H ou Kezuo;Zhang Min;Q u Xiujuan;H u Xuejun;Liu Yunpeng(Department o f Medical Oncolog;Department of Respiratory and Infectious Disease of Geriatrics,Institute of Respiratory Disease,TheFirst Affiliated Hospital,China Medical University,Liaoning Shenyang 110001,China)
出处
《现代肿瘤医学》
CAS
2018年第13期1985-1988,共4页
Journal of Modern Oncology
基金
国家自然科学基金项目(编号:81602098)
辽宁省科技厅项目(编号:2015020457)
2015年度留学人员科技活动项目择优资助(人社厅函[2015]192号)
