摘要
目的:探究水蛭经炮制后的化学成分变化情况。方法:将一批水蛭作为实验对象,对水蛭实施高效液相色谱法(HPLC)-柱后衍生化法炮制,对经过炮制的水蛭中的氨基酸成分予以测定,并对甾体类化合物组成采用气相色谱-质谱联用仪(GC-MS)测定,对炮制前后氨基酸及甾体类化合物的成分变化情况予以比较分析。结果:经过炮制后的水蛭,其氨基酸含量如天冬氨酸、苏氨酸、谷氨酸等成分与炮制前比较明显降低,氨基酸总量由原来的73.93%降低到45.04%,差异存在统计学意义(P<0.05),甾体类化合物组成成分也发生明显变化,胆固醇氧化产物呈现出明显的增加趋势,差异统计学有意义(P<0.05)。结论:水蛭经过炮制后,蛋白质空间构象将会遭到破坏,使氢键断裂,促使氨基酸与甾体类化合物氧化分解,降低氨基酸成分,使甾体类化合物组成成分发生改变,需加强临床关注。
objective：to investigate the chemical composition changes of leech after processing.Methods：our new purchases a batch of Chinese medicine leech as experimental object,the leeches implementation of high performance liquid chromatography（HPLC）-column derivatization method after processing,and after processing of leeches in the amino acid composition was to be measured,and the nonsteroidal compounds by gas chromatography/mass spectrometry（GC-MS）determination of the chemical composition of amino acids and nonsteroidal compounds before and after changes to the comparative analysis.Results：after processing the leeches,and its amino acids such as glutamic acid,aspartic acid,threonine,the composition such as significantly lower compared with before anything,decreased from 73.95% to 73.93%,and the total amino acid differences there was statistical significance（P〈0.05）,nonsteroidal compound composition also had obvious changes,cholesterol oxidation products increased obviously statistically significant（P〈0.05）.Conclusion：leeches after processing,protein conformation will be destroyed,the hydrogen bond rupture,prompt amino acid and nonsteroidal compound oxide decomposition,reduce the amino acid composition,make the nonsteroidal compound composition change,need to strengthen clinical attention.
作者
许石钟
Xu Shizhong(Puyang Hospital of Traditional Chinese Medicine Pharmacy in Henan Province（Puyang 450000)
出处
《陕西中医》
2018年第7期980-982,共3页
Shaanxi Journal of Traditional Chinese Medicine
基金
河南省中医药专项课题(2016ZY3044)
关键词
水蛭
炮制(中药)
色谱法
高压液相
氨基酸类
Leeches Processing （TCD）
Chromatography,high pressure liquid
Amino acids
