摘要
背景:Wnt/β-catenin信号通路在骨关节炎病程中的作用已被大量动物实验和人标本试验验证。Wnt/β-catenin信号通路和基质金属蛋白酶13(matrix metalloproteinases,MMP-13)在骨关节炎的病理过程中都发挥重要作用,两者之间可能有某种潜在的联系。目的:检测β-catenin和MMP-13在骨关节炎滑膜中的表达水平,探讨它们在骨关节炎发病过程中的相关性。方法:骨关节炎组取自行人工膝关节置换术或膝关节镜诊治术骨关节炎患者的关节滑膜组织,共54例;非骨关节炎组取创伤后行截肢术健康青年患者自愿捐赠的正常膝关节滑膜组织,共12例。根据改良的Mankin关节软骨病理评分标准分为非骨关节炎组及轻度、中度、重度骨关节炎组。采用免疫组化检测β-catenin和MMP-13在骨关节炎患者滑膜组织中的表达,并进行相关性分析。结果与结论:(1)MMP-13免疫组化染色结果:非骨关节炎组全部为阴性;轻度骨关节炎组5例阳性、阳性率50%,中度骨关节炎组11例阳性、阳性率65%,重度骨关节炎组23例阳性、阳性率85%;(2)β-catenin免疫组化染色结果:非骨关节炎组1例染色阳性,阳性率为8%;轻度骨关节炎组7例阳性,阳性率70%;中度骨关节炎组14例阳性,阳性率82%;重度骨关节炎组25例阳性,阳性率93%;(3)β-catenin与MMP-13在骨关节炎患者滑膜中的含量呈现明显的正相关关系(r=0.806,P<0.001),其含量亦随着关节退变程度的加重而增加;(4)结果提示,MMP-13和β-catenin在骨关节炎组滑膜组织中均表现为高表达状态,两者在骨关节炎与非骨关节炎组表达差异有显著性意义,并且共同在骨关节炎病程中发挥作用。骨关节炎病变过程中β-catenin和MMP-13均发挥着重要的调控作用,并且MMP-13与β-catenin在参与骨关节炎软骨退变和滑膜炎症反应等病理变化过程中密切相关,这为骨关节炎的靶向基因治疗提供了新的理论方向和实验依据。
BACKGROUND： Role of Wnt/β-catenin signaling pathway in the pathogenesis of osteoarthritis has been proved by numerous animal experiments and human specimen trials. Wnt/β-catenin signaling pathway and matrix metalloproteinase 13（MMP-13） play important roles in pathological process of osteoarthritis, and they may be related with each other. OBJECTIVE： To detect the expression levels of β-catenin and MMP-13 in the synovial tissue of osteoarthritis, and to investigate the relationship between β-catenin and MMP-1 in the pathogenesis of osteoarthritis. METHODS： Synovial tissues were obtained from the 54 patients undergoing total knee arthroplasty or knee arthroscopic diagnosis and treatment surgery and 12 healthy patients suffering from amputation caused by trauma. The samples were divided into non-, mild-, mediumand severe-osteoarthritis groups based on advanced Mankin scores. The expression levels of β-catenin and MMP-13 in the synovial tissues were detected by immunohistochemistry, and correlation analysis was conducted. RESULTS AND CONCLUSION： Immunohistochemistry results showed that MMP-13 was negative in the non-osteoarthritis group, positive in 5（50%） samples in the mild-osteoarthritis group, positive in 11（65%） samples in the medium-osteoarthritis group and positive in 23（85%） samples in the severe-osteoarthritis group. β-Catenin was positive in 1（8%） sample in the non-osteoarthritis group, positive in 7（70%） samples in the mild-osteoarthritis group, positive in 14（82%） samples in the medium-osteoarthritis group and positive in 25（93%） samples in the severe-osteoarthritis group. The expression levels of β-catenin and MMP-13 in the synovial tissues presented a positive correlation（r=0.806, P〈0.01）, and the levels increased with joint degeneration becoming severe. These results imply that there is a significant increase in the expression levels of β-catenin and MMP-13 in the synovial tissues of osteoarthritis compared with the non-osteoarthritis group, and β-
作者
李跃军
朱卫国
方钦正
顾培伦
高鹏
菅永志
羊鹏飞
董金波
Li Yue-jun1, Zhu Wei-guo2, Fang Qin-zheng2, Gu Pei-lun1, Gao Peng1, Jian Yong-zhi1, Yang Peng-fei1, Dong Jin-bo2(1.School of Medicine, Shihezi University, Shihezi 832000, Xinjiang Uygur Autonomous Region, China; 2.Department of Joint Surgery, First Affiliated Hospital of Medical College of Shihezi University, Shihezi 832000, Xinjiang Uygur Autonomous Region, Chin)
出处
《中国组织工程研究》
CAS
北大核心
2018年第16期2472-2477,共6页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金(81160225)
兵团医药卫生专项课题(2013BA020)
兵团国际交流与合作专项基金(2012BC002)
兵团骨科创新团队专项(2014BC003)~~
