摘要
为获得草坪蘑菇圈病原菌拮抗生防菌剂的优化参数,本研究以拮抗蘑菇圈病原-肉褐麟小伞菌(Lepiota brunneo-incarnata)的黄曲霉(Aspergillus flavus)CP01为目的菌,对其分别进行液、固态发酵条件的优化。通过单因素试验优化发酵时间、培养温度、培养基类型及初始pH等条件,对最佳液态发酵条件进行筛选;并通过单因素试验优化培养物料、外源养分、发酵时间和温度等条件,对最佳固态发酵条件进行筛选。结果表明:液体发酵条件下,马铃薯葡萄糖液体培养基(PDB)中菌丝体干重含量最高,而在青秸秆粉浸提液培养基(CSE)中有效菌数最高,且该发酵液对蘑菇圈病原的抑菌效果最佳,培养基最适初始pH为5、最佳发酵条件为30℃、5d;在固态发酵条件下,最适发酵培养物料为经浸提法去营养化的青秸秆粉,外源添加养分为料水比1∶50的青秸秆粉浸提液,最适发酵条件为33℃、9d。以上优化参数为草坪蘑菇圈生防菌剂的研发奠定了基础。
The aim of this study was to optimize the fermentation conditions for obtaining the biological inoculant of an antagonist of the fairy ring mushroomLepiota brunneoincarnata.Aspergillus flavus CP〈01 is a pathogen of the fairy ring mushroom,and we optimized its liquid and solid fermentation conditions.We screened for the best liquid fermentation conditions of the antagonist in terms of medium type,fermentation time,temperature,and initial pH using the single-factor method.We also screened for the optimal solid fermentation conditions of the target host fungus,viz.,L.brunneoincarnata,in terms of training material and additional nutrition type,fermentation time,and temperature using the single-factor method.Results showed that the optimal growth medium of A.flavus was potato dextrose broth（PDB）,which achieved the maximum dry weight of mycelial bead,while the medium type ensuring the maximum number of effective bacteria and the best antibacterial effect was corn stalk leach liquor（CSE）with an optimal initial pH of 5.The optimal liquid fermentation conditions were identified as 30 ℃ and 5 days.The optimal medium type was counteract-eutrophication straw powder by extraction as training material and corn stalk leach liquor at 1∶50 as additional nutrition type for maximum spore yield.The optimal solid fermentation conditions were identified as 33℃and 9 days.These optimized parameters establish a technical basis for the development of an antibiological inoculant of fairy rings.
作者
赵妗颐
李剑峰
张淑卿
何秋婷
Zhao Jin-yi;Li Jian-feng;Zhang Shu-qing;He Qiu-ting(Institute of Soil and Environment Bioremediation in Karst habitats,Guizhou Normal College,Guiyang 550018,Guizhou,China;Key Laboratory for Grassland Ecosystem of Ministry of Education,College of Grassland Science,Gansu Agricultural University,Lanzhou 730070,Gansu,China;College of I.ife Science,Guizhou University,Guiyang 550025,Guizhou,China）（1.InstituteofSoilandEnvironmentBioremediationinKarsthabitats,GuizhouNormalCollege,Guiyang550018,Guizhou,China;2.KeyLaboratoryforGrasslandEcosystemofMinistryofEducation,CollegeofGrasslandScience,GansuAgriculturalUniversity,Lanzhou730070,Gansu,Chin;3.CollegeofLifeScience,GuizhouUniversity,Guiyang550025,Guizhou,China）（1.Institute of Soil and Environment Bioremediation in Karst habitats,Guizhou Normal College,Guiyang 550018,Guizhou,China;2.Key I.aboratory for Grassland Ecosystem of Ministry of Education,College of Grassland Science,Oansu Agricultural University,I.anzhou 730070,Gansu,China 3.College o[ I.{[c Science,Guizhou University,Guiyang 550025,Guizhou,Chin)
出处
《草业科学》
CAS
CSCD
2018年第6期1368-1377,共10页
Pratacultural Science
基金
国家自然科学基金项目(41561072)
贵州省教育厅自然科学研究项目(黔教合KY字[2015]377)
奥林高尔夫教育与研究基金(AL2015001)
关键词
草坪蘑菇圈
拮抗菌
液态发酵
固态发酵
抑菌活性
fairy ring
antagonist
liquid fermentation
solid fermentation
anti bacterial effect
