摘要
[Objectives] The aim was to explore the best parameters for processing Aconiti Kusnezoffii Radix by bean curd. [Methods] Orthogonal test( L_(16)(4~3)) was designed to investigate the optimal soaking time,decocting time and bean curd proportion for processing Aconiti Kusnezoffii Radix by bean curd. The contents of monoester alkaloids and diester-alkaloids were measured as the evaluation indexes. [Results]The contents of monoester alkaloids were 0. 11%,0. 07%,0. 062% and 0. 048% respectively under the four levels of soaking time,were0. 081%,0. 066%,0. 074% and 0. 067% respectively under the four levels of decocting time,and were 0. 070%,0. 072%,0. 080% and0. 073% respectively under the four levels of bean curd proportion. The content of diester-alkaloids was all below 0. 034%. The influence intensity of the three factors on the content of monoester alkaloids ranked as soaking time > decocting time > bean curd proportion. Under any of the above conditions,the content of diester-alkaloids was below the limit of Chinese Pharmacopoeia. The content of diester-alkaloids was reduced obviously with the increased use of bean curd. [Conclusions] The optimum processing conditions for Aconiti Kusnezoffii Radix were A_3B_3C_2,i. e.,soaking time of 6 d,decocting time of 5 h and bean curd proportion of 25%.
[Objectives] The aim was to explore the best parameters for processing Aconiti Kusnezoffii Radix by bean curd.[Methods] Orthogonal test （L 16 （4 3）） was designed to investigate the optimal soaking time, decocting time and bean curd proportion for processing Aconiti Kusnezoffii Radix by bean curd. The contents of monoester alkaloids and diester-alkaloids were measured as the evaluation indexes.[Results] The contents of monoester alkaloids were 0.11%, 0.07%, 0.062% and 0.048% respectively under the four levels of soaking time, were 0.081% , 0.066%, 0.074% and 0.067% respectively under the four levels of decocting time, and were 0.070%, 0.072%, 0.080% and 0.073% respectively under the four levels of bean curd proportion. The content of diester-alkaloids was all below 0.034%. The influence intensity of the three factors on the content of monoester alkaloids ranked as soaking time 〉 decocting time 〉 bean curd proportion. Under any of the above conditions, the content of diester-alkaloids was below the limit of Chinese Pharmacopoeia. The content of diester-alkaloids was reduced obviously with the increased use of bean curd.[Conclusions] The optimum processing conditions for Aconiti Kusnezoffii Radix were A 3B 3C 2, i.e. , soaking time of 6 d, decocting time of 5 h and bean curd proportion of 25%.
