摘要
盐分胁迫会对植物的生理生化特性产生影响，通过测定植物在盐分胁迫下的生理生化指标，是评估其对盐害耐受能力的方法之一。以3个实用桑树（MorusL．）品种的绿枝扦插盆栽苗及其离体叶片为材料，分别用300mmol／LNaC1溶液进行盐胁迫处理，观察与测定3个品种的扦插苗在盐胁迫下的叶片形态、渗透调节物质含量和抗氧化酶活性。盐胁迫处理后3个品种扦插苗叶片的失绿程度从小到大依次为大10、金10、育711号。测定盐胁迫处理后4d3个品种扦插苗根、皮、叶中的渗透调节物质含量和抗氧化酶活性均存在显著差畀：3个品种的根、皮和叶中的脯氨酸（Pro）含量均升高，且育711号和金10的增幅显著高于大10；育711号的皮和叶中的可溶性蛋白含量最高，金10最低，而在根中的可溶性蛋白含量则是金10最高，大10最低；育711号叶中的超氧化物歧化酶（SOD）活性极显著高于金10和大10，而大10根和皮中的SOD活性相对较低；育711号和金10根中的过氧化物酶（POD）活性显著高于大10，而大10叶中POD具有较高的活性；育711号根中的丙二醛（MDA）含量增幅显著高于金lO和大10。研究结果表明，在盐胁迫下，大10扦插苗根和叶中的渗透调节物质含量和抗氧化酶活性均较为稳定，叶圆片失绿程度最低，而育711号不同组织渗透调节物质的积累显著增加，抗氧化酶活性提高。据此评价3个桑树品种对盐害的耐受能力由高到低依次为大10、金10、育711号。
Determining the physiological and biochemical indexes of plant under salt stress is one of the methods to eval- uate the salt tolerance of plant. Potted plantlets generated from greenwood cuttings and in vitro leaves of three practical mulberry varieties were treated with 300 mmol/L NaCI solution. The foliation under salt stress was observed and the con- tent of osmotic regulative substances and antioxidase activity were measured. The loss degree of green coloration under salt stress in the leaf of these three varieties was in the ascending order of Da 10, Jin 10 and Yu 711, After 4 d under salt stress, the content of osmotic regulative substances and antioxidase activity in root, bark and leaf of different mulberrv varieties had significant difference. After NaCI treatment, the contents of proline in different organs of three mulberry varieties were all increased and the increment of proline content in Yu 711 and Jin 10 was significantly higher than that in Da 10. The soluble protein content in bark and leaf was the highest in Yu 711 and the lowest in Jin 10. The soluble protein content in root was the highest in Jin 10 and the lowest in Da 10. The superoxide dis-mutase （SOD） activity in leaf of Yu 711 was significantly higher than that in Jin 10 and Da 10. Besides, the SOD activity in root and bark of Da 10 was kept at a low level. The peroxidase （POD） activity in root of Yu 711 and Jin 10 was signifi- cantly lower than that of Da 10, whereas it was kept at a high level in leaf of Da 10. The increment of malondialdehyde （MDA） content in root of Yu 711 was significantly higher than that in Da 10 and Jin 10. These results indicated that the content of osmotic regulative substances and antioxidase activity in root and leaf of Da 10 were steadier under salt stress, and the loss degree of green coloration was the lowest. The content of osmotic regulative substances and antioxidase ac- tivity in different tissues of Yu 711 were increased. It was suggested that the salt tolerance from high to low were in t
作者
殷朝瑞
方荣俊
尚春琼
沈萩荻
曹旭
程嘉翎
Yin Chaorui;Fang Rongjun;Shang Chunqiong;Shen Qiudi;Cao Xu;Cheng Jialing(Colloge of Biotechnology,Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212018,China;Key La- boratory of Silkworm and Mulberry Genetic Improvement,Ministry of Agriculture,Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang Jiangsu 212018,China)
出处
《蚕业科学》
CAS
CSCD
北大核心
2018年第3期359-366,共8页
ACTA SERICOLOGICA SINICA
基金
国家自然科学基金项目(No.31700527)
现代农业产业技术体系建设专项(No.CARS-18)
关键词
桑树
扦插苗
盐胁迫
渗透调节物质
抗氧化酶
耐盐性品种
Morus L.
Cutting plantlet
NaCl stress
Osmotic regulative substance
Antioxidase
Salt-tolerance variety
