摘要
为了考察小檗碱(BBR)及其衍生物(A4,B4)在体内运输过程中与生物体大分子互相作用的作用机制及能量转移方式,文章通过荧光光谱分析方法考察了相同浓度不同温度(15,25,37℃)条件下的小檗碱衍生物对蛋白产生的猝灭作用。结果表明随着化合物浓度的增加,BSA均显示出明显的下降趋势,说明BBR、A4和B4与牛血清白蛋白相互作用表现出强的荧光猝灭,根据下降程度可判断出结合能力的顺序为B4>A4>BBR。根据Stern-Volmer方程得出猝灭常数Ksv随温度的升高而降低,说明小檗碱及其衍生物与牛血清白蛋白发生的荧光猝灭方式为静态猝灭。通过荧光光谱和紫外光谱分析方法连用以及根据Forster非放射性能量转移理论,计算得出BBR、A4和B4与牛血清白蛋白之间的结合距离分别为3.53,3.38,3.27 nm,表明BBR、A4、B4与BSA之间发生的是非辐射能量转移。
In order to investigate the mechanism and energy transfer of interaction between berberine（ BBR）,its derivatives（ A4,B4） and macromolecules in vivo,in this study,the quenching effect of BBR,A4,B4 with protein at the same concentration and different temperatures（ 15,25,37 ° C） by fluorescence spectroscopy was investigated. The consequences showed that with the increase of compound concentration,the interaction between BBR,A4,B4 and bovine serum albumin（ BSA） showed strong fluorescence quenching,and the order of binding ability was B4〉A4〉BBR based on the degree of quenching. According to the SternVolmer equation,the quenching constant Ksvdecreased with temperature increasing,indicating that the fluorescence quenching mode of BBR,A4,B4 with BSA was static quenching. By fluorescence spectroscopy and UV spectroscopy together with Forster non-radioactive energy transfer theory,the binding distances between BBR,A4,B4 and BSA were calculated respectively to be 3. 53,3. 38,and3. 27 nm,showing that the energy transfer between BBR,A4,B4 and BSA was non-radiative energy transfer.
作者
赵潇俊
夏利华
王孟可
黄怡
黄维维
蔡星晨
王文超
李庆勇
ZHAO Xiao-jun;XIA Li-hua;WANG Meng-ke;HUANG Yi;HUANG Wei-wei;CAI Xing-chen;WANG Wei-chao;LI Qing-yong(College of Pharmacy,Zhefiang University of Technology,Hangzhou 310014,China)
出处
《药物生物技术》
CAS
2018年第3期206-209,共4页
Pharmaceutical Biotechnology
基金
国家自然科学基金(No.31270397)
浙江省钱江人才项目
