摘要
目的:观察针刺心经经穴对心肌缺血大鼠下丘脑室旁核神经元电活动特征的影响,探讨针刺心经抗心肌缺血效应的下丘脑调控机制。方法:随机选取8只SD大鼠作为伪手术组,其余采用冠状动脉左前降支结扎法复制心肌缺血模型。将模型复制成功的大鼠随机分为模型组、电针心经组和电针肺经组,每组8只。电针心经组电针"神门"-"通里"段,电针肺经组电针"太渊"-"列缺"段,刺激20min,1次/d,共7d。将微电极阵列植入大鼠下丘脑室旁核,采用Plexon多通道采集系统记录神经元放电和场电位;利用Offline Sorter软件进行神经元信号聚类分析,筛选神经元放电信号;采用Neuro Explorer软件对神经元的放电波形进行自相关与互相关分析,并分析各组神经元信号的频率、特征和实时频谱。结果:神经元信号聚类分析和自相关分析可见,伪手术组大鼠下丘脑室旁核区可见2个中间神经元活动,模型组可见2个中间神经元放电活跃,电针心经组可见4个神经元放电活动,其中中间神经元1个、锥体神经元3个,电针肺经组可见1个中间神经元放电活动。互相关分析可见,电针心经组SPK 02a和SPK 02b神经元放电活动之间存在抑制关系。神经元放电序列和频率分析可见,与伪手术组比较,模型组大鼠下丘脑室旁核神经元总放电频率明显增强(P<0.01);与模型组比较,电针心经组大鼠下丘脑室旁核神经元总放电频率显著下降(P<0.01);电针肺经组大鼠下丘脑室旁核神经元总放电频率明显高于电针心经组(P<0.01)。实时频谱分析显示电针心经组局部场电位频谱能量显著低于模型组和电针肺经组。结论:针刺心经经穴可显著降低下丘脑室旁核中间神经元活动,激活投射到下丘脑室旁核的锥体神经元活动,且中间神经元与锥体神经元之间存在抑制关系,可能是针刺心经抗心肌缺血效应的重要机制之一。
Objective To observe the effect of electroacupuncture（EA）stimulation of＂Shenmen＂（HT 7）-＂Tongli＂（HT 5）segment of the Heart Meridian on neuronal electrical activities of hypothalamic paraventricular nucleus（PVN）in rats with myocardial ischemia（MI）,so as to investigate its possible mechanism underlying improvement of MI.Methods Thirty-two SD rats were randomly divided into sham control,model,HT 7-HT 5 and＂Taiyuan＂（LU 9）-＂Lieque＂（LU 7）groups（n=8 in each group）.EA preconditioning（2 Hz,1 V,20 min）was applied to bilateral HT 7-HT 5 and bilateral LU 9-LU 7,respectively,once everyday for 7 days.The electrical activities of the right PVN region were recorded by the implanted microelectrode array（2×4）and Plexon multi-channel acquisition system.Cluster analysis of neuronal signals was carried out by Offline Sorter software.The discharge waveforms,autocorrelation and cross-correlation of neuronal activities were analyzed by using Neuro Explorer software.Results Cluster analysis of neuronal signals showed that 2,2,1 and 1 interneuron in the sham,model,HT 7-HT 5,and LU 9-LU 7 groups,and 3 pyramidal neurons in the HT 7-HT 5 were acquired.Cross correlation analysis showed that the SPK 02 aand SPK 02 bneurons of the HT 7-HT 5 group had an inhibitory relationship.The total discharge frequency was significantly increased in the model group relevant to the sham group（P〈0.01）,and was markedly lower in the HT 7-HT 5 group than in the model group and LU 9-LU 7 group（P〈0.01）.Real-time spectrum analysis showed that the local field potential spectrum energy of the HT 7-HT 5 group was significantly lower than that of the model group and the LU 9-LU 7 group.Conclusion EA of HT 7-HT 5 segment of the Heart Meridian can inhibit the electrical activity of interneuron and activate the electrical activity of pyramidal neuron in PVN region,and an inhibitory relationship exists between the interneuron and pyramidal neuron in MI rats,which may be a mechanism of EA in regulating acti
作者
蔡荣林
崔帅
吴子建
何璐
余情
王洁
彭传玉
胡玲
周逸平
CAI Rong-lin;CUI Shuai;WU Zi-jian;HE Lu;YU Qing;WANG Jie;PENG Chuan-yu;HU Ling;ZHOU Yi-ping(Research Institute of Acupuncture-moxibustion and Meridians,Anhui University of Traditional Chinese Medicine,Hefei 230038,Chin)
出处
《针刺研究》
CAS
CSCD
北大核心
2018年第7期406-413,共8页
Acupuncture Research
基金
国家自然科学基金项目(No.81774414)
安徽省高校优秀青年人才支持计划重点项目(No.gxyqZD 2016134)
安徽高校科研创新平台建设项目(No.2015TD 033)
安徽省高校自然科学重点项目(No.KJ 2016A410)
关键词
电针
心经
心肌缺血
下丘脑室旁核
神经元活动
微电极阵列
Electroacupuncture
Heart Meridian
Myocardial ischemia
Hypothalamic paraventricular nucleus
Neuronal activity
Microelectrode array
