摘要
目的构建了基于高荧光碳量子点测定人发中痕量汞离子的开-关荧光探针。方法利用在碳量子点里掺杂氮、硫原子来提高碳量子点的荧光量子产率,掺杂后的碳量子点对汞离子具有特殊识别功能,可以选择性和汞离子配位络合,通过检测加入汞离子前后碳量子点荧光强度的变化测定样品中汞离子。结果当汞离子浓度在0～0.30μΜ范围内时,其浓度与碳量子点的荧光强度有着很好的线性关系,检出限为1.37nM,明显低于美国环保机构制定的饮用水中汞离子最大允许浓度(10nM)。将其用于人发中痕量汞离子检测,本方法测定的结果和原子吸收分光光度法测得的结果相一致,且汞离子的回收率分别为96.8%～98.1%,98.6%～104.5%,97.7%～105.3%(RSD<3.4%)。结论该方法具有操作简单、快速、灵敏度高、特异性好、无需大型仪器、成本低等优点,适用于人发中痕量汞离子的测定。
Objective To construct an on-off fluorescent probe for determination of trace mercury in human hair based on the highly fluorescent carbon quantum dots. Methods Doping nitrogen,sulfur impurity atoms in carbon quantum dots can increase the fluorescent quantum yield,which have special recognition to mercury ions and can selectively coordinated with mercury ions;through determination of the fluorescence intensity before and after adding mercury ions in the carbon quantum dots mercury ions can be measured in samples. Results The fluorescence intensity displays a good linear response with Hg2＋ concentration in the range of 0～0.30μM and the detection limit is 1.37 nM. The value is obviously lower than that of the maximum level of Hg2＋ ions permitted（10.0 nM） by the Environmental Protection Agency（USA） for drinking water. The results of applying the method to measure the mercury ions in human hair samples were consistent with those by atomic absorption spectrometry and the recovery rates of mercury ions were 96.8%～98.1%,98.6%～104.5%,97.7%～105.3%（RSD3.4%）,respectively. Conclusion The method is simple,fast,high sensitively,good specificity,no large-scale equipment,low-cost,et al. which is applicable to determination of trace mercury ion in human hair.
作者
贺建同
傅政
HE Jiantong;FU Zheng(Xinxiang maternal and child health hospital,Xinxiang Henan 453000,Chin)
出处
《实验与检验医学》
CAS
2018年第3期300-304,共5页
Experimental and Laboratory Medicine
基金
国家自然科学基金(Nos.30970696
21172056)
河南省科技发展重大项目(No.142102310273)
关键词
掺杂
荧光
碳量子点
头发
荧光发射光谱
Doping
Fluorescence
Carbon quantum dots
Hair
Fluorescence emission spectra
