摘要
目的使用运动及细颗粒物(PM2.5)暴露干预手段,观察大鼠肝脏脂肪代谢功能,探讨其对大鼠肝脏的影响机制,为PM2.5暴露造成机体损伤提供理论支持。建立PM2.5暴露大鼠运动模型。方法选取2017年3—5月在山西医科大学公共卫生学院实验室,进行10周训练的雄性SD大鼠40只,采用随机数字表法分为4组:安静对照组(C)10只、运动组(S)10只、PM2.5暴露组(E)10只、PM2.5暴露运动组(ES)10只。采用气管滴注对动物进行染毒[(6 mg/(kg·b·w)],使用GPO-PAP法测定TG,微板法测定NEFA。结果运动组TG值为(9.908±1.908)mmol/gprot,NEFA值为(88.402±5.500)μmol/gprot],均小于其他3组,与安静对照组相比,运动组TG、NEFA差异有统计学意义,PM2.5暴露组两项指标均差异有统计学意义;与PM2.5暴露组相比,PM2.5暴露运动组各项指标[TG:(28.340±4.741)mmol/gprot;NEFA:(91.823±5.145)μmol/gprot]均差异有统计学意义;通过析因分析,提示运动与PM2.5暴露存在交互作用,进一步回归分析得出,染毒效应强于运动,运动将减弱毒效应。结论 PM2.5暴露影响大鼠肝脏脂肪代谢功能,运动与PM2.5暴露存在交互作用,该作用与运动调节大鼠肝脏功能有关。
Objective To observe the liver fat metabolic function by the exercise and PM2.5 exposure means thus providing theoretical support for the organ injury of PM2.5 exposure and establishing the PM2.5 exposure rat motor model.Methods 40 male SD rats in our hospital from March to May 2017 were selected for 10-week training and randomly divided into four groups with 10 cases in each, the silent control C group, the exercise group S group, the PM2.5 exposure group and PM2.5 exposure and exercise group ES GROUP, and the animals were for contamination 6 mg/kg·（b·w·by） the intratracheal instillation, and the TG was measured by the GPO-PAP method, and the NEFA was tested by the microplate test. Results The TG value and NEFA value in the exercise were respectively（9.908±1.908）mmol/gprot and（88.402±5.500）μmol/gprot, which were lower than those in the other three groups, and there were difference in the TG and NEFA between the silent control group and the exercise group, and the differences in the two indicators in the PM2.5 exposure group were obvious, and the difference in the TG in the PM2.5 exposure and exercise group was statistically significant, and the differences in the various indicators between the PM2.5 exposure group and the PM2.5 exposure and exercise group were obvious [TG：（28.340 ±47.41）mmol/gprot;NEFA：（91.823 ±5.145）μmol/gprot], and the analysis showed that the motor and PM2.5 exposure were interacted, and the further regression analysis showed that the contamination efficacy was stronger than that of exercise, and the exercise could reduce the toxic and side effects.Conclusion PM2.5 exposure affects the liver fat metabolic function of rats, and the exercise is interacted with the PM2.5 exposure, and the effect is related to the adjustment of rat livers by exercise.
作者
许磊
XU Lei(School of Public Health,Shanxi Medical University,Taiyuan,Shanxi Province,030001 China;School of Sports in stitute,Shanxi University of Finance and Economics,Taiyuan,Shanxi Province,030001 China)
出处
《系统医学》
2018年第4期19-21,共3页
Systems Medicine
基金
山西省体育局科研课题资助项目(17TY133)
山西财经大学青年科研基金项目资助课题(Z06042)
