摘要
目的 应用高效液相色谱-电喷雾-离子阱质谱法分析雾灵乌头不同部位提取物的生物碱。方法 采用Agilent XDB-C18色谱柱（250 mm×4.6 mm，5 μm），以0.1%氨水-乙腈溶液为流动相，梯度洗脱，流速为1 ml/min，柱温为30 ℃；正离子模式采集。结果 雾灵乌头根部发现61种生物碱，鉴别出46种。在茎中发现38种生物碱，鉴定出33种，有27种与根部相同。在叶中检测出18种生物碱，其中8种与根部生物碱相同。结论 该方法精确、可靠、高效，适用于雾灵乌头成分的快速鉴定，为雾灵乌头的开发利用及阐明其药效物质基础提供参考。
Objective To analyze the alkaloids in the different parts of Aconitum wulingense by HPLC-ESI-Trap-MS. Methods The Agilent XDB-C18（250 mm×4.6 mm, 5 μm） column with gradient elution of 0.1% solvent （A）-acetonitrile（B）, at a flow rate of 1 ml/min was used. The column temperature was set at 30 ℃. The MS analysis was based on positive ions mode. Results In the roots, a total of 61 diterpenoid alkaloids were discovered, among which 46 were identified. In the stems, 38 alkaloids have been found, among which 33 alkaloids were identified and 27 were the same with the roots. In the leaves, 18 alkaloids have been detected and 8 were the same with the roots. Conclusions The method is accurate, reliable and efficient, and is suitable for rapid identification of ingredients in Aconitum wulingense, which provides a reference for the development and utilization of Aconitum wulingense and clarify its efficacy and material basis.
作者
郭晓宇
张伽妹
全庆华
姬瑞芳
谭鹏
刘永刚
Guo Xiaoyu;Zhang Jiamei;Quan Qinghua;Ji Ruifang;Tan Peng;Liu Yonggang(Master of Grade 2017,School of Chinese Materia Medical of Beo＇ing University of Chinese Medicine,Beijing 102488,China)
出处
《国际中医中药杂志》
2018年第7期633-638,共6页
International Journal of Traditional Chinese Medicine
