摘要
目的观察阻塞性睡眠呼吸暂停(OSA)患者–A930G、C242T基因多态性与认知功能障碍的相关性,探讨由CYBA编码的p22phox等位基因在合并有认知功能障碍的OSA患者中的作用。方法选择2014年9月至2016年11月于呼吸睡眠科就诊的男性非吸烟OSA患者,其中认知功能障碍OSA患者157例,无认知功能障碍OSA患者526例。对患者进行认知功能评估、多导睡眠监测及遗传分析,ELISA法测定还原型烟酰胺腺嘌呤二核苷酸磷酸氧化酶(NOX)活性和尿8-羟基脱氧鸟苷(8-OH-d G)水平。结果–930G等位基因携带者频率在两组受试者中差异无统计学意义(P>0.05)。无认知功能障碍的OSA受试者TT/CT基因型频率明显升高(P<0.05)。认知功能障碍的OSA受试者NOX活性较高(P<0.01),无等位基因T突变的受试者具有更高的NOX活性(P<0.05)。认知功能障碍的OSA受试者尿8-OH-d G水平较高(P<0.05),无等位基因T变异的受试者尿8-OH-d G水平更高(P<0.05)。结论 p22phox C242T多态性可能参与了OSA合并认知功能障碍氧化应激的发生发展。
Objective To analyze a possible association of-A930 G and C242 T polymorphism with cognitive dysfunction in obstructive sleep apnea（OSA） patients, and assess potential interactions of CYBA alleles in OSA patients with cognitive dysfunction. Methods A total of 157 OSA patients with cognitive dysfunction were recruited as an experimental group, and 526 matched OSA patients without cognitive dysfunction as an control group. The neurocognitive assessment, polysomnography, genetic analyses, NADHP oxidase（NOX） activity, determination of urinary 8-OH-d G were completed in all subjects. Results Frequencies of the-930 G allele carriers were not significantly different between two groups（P〈0.05）. Frequencies of the TT/CT genotypes were significantly higher in the OSA patients without cognitive dysfunction（P〈0.05）. NOX activity was assessed and found to be increased in the OSA patients with cognitive dysfunction（P〈0.01）. NOX activity was significantly higher in whom the allelic T variant was absent（P〈0.05）.The level of urinary 8-OH-d G was higher in the OSA patients with cognitive dysfunction（P〈0.05）. The level of urinary8-OH-d G was significantly higher in whom the allelic T variant was absent（P〈0.05）. Conclusion The p22 phox C242 T polymorphism may be involved in the development of oxidative stress reaction in OSA patients with cognitive dysfunction.
作者
姜良征
欧阳松云
苏皎
JIANG Liangzheng;OUYANG Songyun;SU Jiao(Department of Respiratory and Sleep-related Diseases, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P. R. Chin)
出处
《中国呼吸与危重监护杂志》
CAS
CSCD
北大核心
2018年第4期373-376,共4页
Chinese Journal of Respiratory and Critical Care Medicine
基金
河南省科技厅重点项目(162102310293)
