摘要
目的以实验室前期制备的葛根素微乳为基础,采用乳糜微粒阻断法研究食物中脂质对葛根素微乳经淋巴转运的影响。方法以环己酰亚胺为乳糜微粒阻滞剂阻断药物经淋巴转运,HPLC法测定大鼠血浆中葛根素浓度,比较阻断组与未阻断组生物利用度来计算淋巴转运的比例。结果葛根素微乳在脂质处理组的淋巴转运比例与水处理组不存在显著性差异,但证实了微乳对促进葛根素淋巴转运及提高其生物利用度的重要贡献。ig给予脂质不能进一步促进葛根素微乳的经淋巴转运,但证实了葛根素以微乳为载体能降低食物中脂质对其口服生物利用度的影响。结论食物中的脂质并未进一步促进葛根素微乳淋巴转运,且对生物利用度影响不大。
Objective To study the effects of lipid-rick food on lymphatic transport absorption of puerarin microemulsion based on the puerarin microemulsion prepared before. Methods Cycloheximide was used to block lymphatic transport of the microemulsion, and the concentration of puerarin in plasma was determined by HPLC. Bioavailability of blocking group and unblocking group were compared to calculate the proportion of puerarin absorbed by lymphatic transport. Results Administration of lipid did not enhance the proportion of puerarin absorbed by lymphatic transport, compared to administration of water as controls, but microemulsion formulation contribute to the improved lymphatic transport and bioavailability of puerarin. Besides, administration of lipid failed to further increase the lymphatic transport of puerarin microemulsion, but it was evidenced that microemulsion formulation could decrease the effects of lipid-rich food on bioavailability of oral drugs. Conclusion Lipid in food could not enhance the lymphatic transport of puerarin microemulsion and show no significant effects on the bioavailability.
作者
李泳江
吴军勇
杨苏芳
胡雄彬
刘新义
向大雄
LI Yong-jiang;WU Jun-yong;YANG Su-fang;HU Xiong-bin;LIU Xin-yi;XIANG Da-xiong(Department of Pharmacy, Second Xiangya Hospital, Central South University, Changsha 410011, China;Hunan Translational Medicine and Innovative Drug Engineering Technology Research Center, Changsha 410011, China;Institute of Clinical Pharmacy, Central South University, Changsha 410011, China)
出处
《中草药》
CAS
CSCD
北大核心
2018年第12期2925-2930,共6页
Chinese Traditional and Herbal Drugs
基金
湖南省科技计划项目(2016TP2002)
关键词
食物
脂质
淋巴转运
葛根素
微乳
生物利用度
food
lipid
lymphatic transport
puerarin
microemulsion
bioavailability
