摘要
目的探讨大鼠肝缺血再灌注损伤机制。方法建立SD大鼠肝缺血再灌注损伤模型，随机分为实验组（n=75）和对照组（n=75）。采用酶联免疫吸附试验（ELISA）法和Western blot法检测大鼠血清和肝脏组织白细胞介素-1（IL-1）表达水平和细胞间黏附分子-1（ICAM-1）、核因子-κB（NF-κB）的含量。结果（1）实验组和对照组肝缺血冉灌注后1、3、6h的IL-1的水平分剧为（90．45±14．76）、（101．19±10．84）、（116．77±13．87）pg／ml；（71．06±10．71）、（78．77±20．41）、（91．19±26．42）pg／ml。两组差异有统计学意义（P=0．000）；（2）Western blot图谱分析，随着时间的延长，肝匀浆组织ICAM-1和NF-κB蛋白的含量逐渐增多，且实验组明显高于对照组。结论肝脏组织中IL-1、ICAM-1的表达增加与大鼠肝缺血再灌注损伤有关；其机制与通过激活NF-κB上调，对ICAM-1和IL-1表达调控加强有关。
Objective To study the mechanism of hepatic ischemiareperfusion injury in rats. Methods SD rat liver ischemia - reperfnsion injury model was established. The animals were randomly divided into experimental group and control group. The contents of serum interleukin - 1 （ IL - 1 ） and intereellular adhesion molecule - 1 （ ICAM - 1 ） and nuclear factor - κB （ NF - κB） in liver homogenate tissue were measured by enzyme linked immunosorbent assay （ELISA） and Western blotting. Results （ ] ） The levels of IL - 1 in the experimental group and the control group at 1 h, 3 h, and 6 h after operation were （90.45 ± 14.76）, （101.19 ± 10.84）, （116.77 ± 13.87） pg/ml, and （71.06 ± 10.71）, （78.77 ± 20. 41 ） and （91.19 ± 26. 42 ） pg/ml respectively with the difference being significant between the two groups （P = 0. 000）. （2） Western blotting analysis showed that the eontents of ICAM - 1 and NF - κB protein in liver homogenate tissue were gradually increased with time, and those in the experimental group were significantly higher than in the control group. Conclusion The increased expression of IL - 1 and ICAM - 1 in liver tissue is related to the hepatic ischemia - reperiusion injury in rats, which may be related to the up - regulation of ICAM - 1 and IL - 1 expression through activation of NF -κB.
作者
李伟涛
翟淑萍
苑伟
张北平
章小兵
王百林
Li Weitao;Zhai Shuping;Yuan Wei;Zhang Beiping;Zhang Xiaobing;Wang Bailin(Department of General Surgery, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou 510220, China （ Li WT, Zhai SP, Yuan W, Wang BL;The First Medical College of Guizhou Medical University, Guiyang 550004, China （ Zhang BP, Zhang XB)
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2018年第7期1253-1255,共3页
Chinese Journal of Experimental Surgery
基金
广东省自然科学基金（2015A03313364）
