摘要
目的研究四逆散对创伤后应激障碍(PTSD)模型大鼠海马区神经元结构的影响。方法用随机数表法将SD雄性大鼠随机分为5组,每组10只:空白对照组、模型组、阴性对照组、阳性对照组和实验组。阴性对照组大鼠于造模前1 h灌胃给予0.9%Na Cl;阳性对照组大鼠灌胃给予盐酸帕罗西汀溶液4.20mg·kg^(-1),实验组大鼠灌胃给予四逆散水煎液2.41 g·kg^(-1)。每日灌胃1次,灌胃容积10 m L·kg^(-1),共计7 d。除了空白对照组以外,其余4组动物均复制PTSD模型。造模结束后,各组大鼠立即心脏灌注并采集海马样品,在1万倍电镜下观察并比较各组大鼠的海马CA1区、CA3区超微结构差异性。结果空白对照组的海马CA1、CA3区神经元数量较多,体积较大,细胞结构清晰;细胞核圆形或椭圆形,核膜光滑、完整,核内染色质分布均匀,常染色质丰富,核仁可见。与空白对照组比较,模型组的神经元明显损伤,表现为神经元数量较少,细胞明显肿胀,电子密度降低;细胞核圆形,核内染色质结构松散,偶见核仁,提示造模后大鼠海马CA1、CA3区神经元明显损伤。与模型组比较,阴性对照组变化与其相似,提示0.9%Na Cl灌胃对大鼠没有造成明显的应激影响。而阳性对照组和实验组的神经元明显恢复,且2组变化基本类似,表现为神经元密集排列,细胞大小不一,细胞结构清晰;细胞核多圆形,核膜光滑、完整,核内染色质分布均匀,常染色质丰富,核仁可见。结论四逆散可以改善PTSD及睡眠障碍大鼠海马区CA1、CA3神经元形态,进而正向调节PTSD大鼠状态的恢复。
Objective To study the regulation of Sinisan on the faction of the ultra structure of hippo-campus in rats with post-traumatic stress disorder（ PTSD）. Methods SD rats were divided equally into 5 groups（ each group had 10 rats） ： blank control group,model group,negative control group,positive control group and experimental group. The control blank group did not copy the model,do not receive treatment,the normal feeding. Model group,repetitive PTSD model was induced by current stimulation,but not treated. Negative control group,equal volume of0. 9% Na Cl. Paroxetine group（ paroxetine hydrochloride 0. 42 mg·m L-1） and experimental group（ Sinisan,containing crude drug0. 24 g·m L-1）. The rats was given to drugs 1 h before the model establishment. The rats were administered with 10 m L·kg-1,once a day,for a total of 7 d. In each group,the heart was perfused and the hippo-campus tissue was collected. The changes of ultra structure of CA1 and CA3 area in hippo-campus of rats were observed by transmission electron microscope. Results In the blank control group,the number of neurons in hippo-campus CA1 and CA3 area was larger,the volume was larger and the cell structure was clear,and the nucleus was round or oval,the nuclear membrane was smooth and complete,and the distribution of chromatin in the nucleus was uniform. Compared with the blank control group,the neurons in the model group were obviously damaged,which showed that the number of neurons was less,the cells were swelling,and the electron density was decreased. The nucleus was round,the chromatin structure was loose,and the nucleolus was occasionally seen. It was suggested that the hippocampal CA1 and CA3 neurons in the hippocampus of rats were obviously damaged. The model group was similar to the negative control group,which indicated that there was no obvious stress effect on the rats after intragastric administration of normal saline. Positive control group and experimental group of neurons were restored,and the two groups showed similar cha
作者
李越峰
曹瑞
牛江涛
边甜甜
司昕蕾
严兴科
LI Yue- feng;CAO Rui;NIU Jiang - tao;BIAN Tian - tian;SI Xin - lei;YAN Xing - ke(a College of Pharmacy, b. Key laboratory of Quality and Standard of Traditional Chinese Medicine in Gansu Province, Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2018年第13期1561-1564,共4页
The Chinese Journal of Clinical Pharmacology
基金
国家自然科学基金资助项目(81460611)
国家教育部科学技术研究重点基金资助项目(212186)
甘肃省自然科学基金资助项目(2010:1010RJZA212
2014:145RJZA076)
甘肃省财政厅高校基本科研业务费专项基金资金项目(2013-2)
甘肃省中医药管理局科研课题基金资金项目(GZK-2015-57)
兰州市科技计划基金资金项目(2014-1-188)
关键词
四逆散
创伤后应激障碍
海马
透射电镜
Sinisan
post - traumatic stress disorder
hippocampus
transmission electron microscope
