摘要
目的探讨转谷氨酰胺酶2C多肽(TGM2)在过敏性紫癜(HSP)肾损伤中的表达及其临床意义。方法采用实时荧光定量PCR检测HSP患儿(HSP组,20例)、HSPN患儿[HSPN组,20例,其中HSPN(Ⅱ,ISKD)4例,HSPN(Ⅲ,ISKD)12例,HSPN(Ⅳ,ISKD)4例]和健康对照组20例外周血有核细胞TGM2mRNA的表达;应用免疫组织化学的方法检测HSPN组患儿和对照组(5例)肾组织TGM2蛋白的表达。结果 HSPN组外周血TGM2mRNA表达高于健康对照组和HSP组(P<0.05),健康对照组和HSP组外周血TGM2mRNA表达水平比较差异无统计学意义(P>0.05)。HSPN组和对照组肾组织的表达差异有统计学意义(P<0.01),且TGM2的表达水平与HSPN病理分级严重程度有关联,即病理分级越重,TGM2的表达越强(P<0.001)。结论 TGM2的表达与HSP肾脏损害有关,且与肾脏损害病理程度有关,TGM2可能参与了HSPN的发病机制。
Objective To investigate the expression and clinical significance of transglutaminase 2（TGM2）in Henoch-Schonlein purpura nephritis（HSPN）.Methods The mRNA expression of TGM2 in peripheral blood was detected by real-time PCR in 20 children with Henoch- purpura（HSP）,20 children with HSPN（ISKD grade Ⅱin 4,grade Ⅲin 12 and grade Ⅳin 4）,and 20 healthy controls.The protein expression of TGM2 in kidney tissue was measured by immunohistochemistry in HSPN children and 5 healthy controls.Results The mRNA expression of TGM2 in peripheral blood in HSP control group was lower than that in control group or HSPN group（P〈0.05）.There was a significant difference between control group and HSPN group in renal TGM2 protein expression（P〈0.01）,but not in peripheral blood TGM2 mRNA expression（P〉0.05）.The expression of TGM2 was positively correlated with the pathological HSPN grades（P〈0.001）.Conclusion The expression of TGM2 plays an important role in HSPN and is associated with renal pathological grades,suggesting that TGM2 may be involved in the pathogenesis of HSPN.
作者
徐锦
杨文萍
李群
傅睿
XU Jin;YANG Wen-ping;LI Qun;FU Rui(Medical Department of Graduated School of Nanchang Universit;a. Department of Pahology , 2b. Department of Nephrology , Jiangxi Provincial Children ＇s Hospital, Nanchang 330006, China)
出处
《南昌大学学报（医学版）》
CAS
2018年第2期42-45,共4页
Journal of Nanchang University：Medical Sciences
基金
江西省卫生计生委科技计划(20165480)
