摘要
目的:建立新型肝脏肿瘤射频消融治疗后复发与转移的预测方法。方法:介入治疗进展期肝细胞癌术后获得患者肿瘤组织标本,选取组织标本外围性状较好的部分,处理/加工成1~2 mm3的组织微块,接种于SCID鼠肝脏原位,在动物饲养过程中采用B超对肿瘤组织在肝脏的生长进行探查。待动物肝脏区域形成B超低回声的病灶时,收集动物获取肝脏组织,对其进行H&E染色与Masson染色的病理学分析,观察小鼠肝脏原位肿瘤的界限与包膜完整性、侵润性生长、肿瘤血管生成与是否有转移、侵袭等组织学特征。结果:H&E染色能够对肿瘤的性状进行初步判断,Masson染色能够对肿瘤细胞、微血管及纤维等结构进行分析,确定肿瘤组织的侵润性生长、肿瘤血管生成与血道转移情况,反映出临床标本中肝细胞癌细胞的生物学特性,为患者复发与转移的风险预测提供依据。结论:建立了肝脏肿瘤介入治疗后复发与转移预测方法,有望为患者预后预测提供参考。
Objective： To develop a novel method to predict the potential recurrence or metastasis of hepatocellular carcinoma（HCC） after transcatheter arterial chemoembolization（TACE） treatment. Methods： Clinical specimens were obtained from patients received TACE treatment. Tissues were seeded in SCID mice＇s liver organ. The intrahepatic growth of HCC in mice＇s liver was identified by ultrasonic detection. When HCC cells formed nodule in mice＇s liver organ, samples were collected and analyzed by HE or Masson staining. Results： The infiltrative growth, tumor angiogenesis, or metastasis of HCC cells in mice＇s liver were identified by HE or Masson staining.Conclusion： This work developed a novel method to be potentially palpitated in predicting the recurrence or metastasis of HCC post TACE treatment.
作者
孙慧伟
谢辉
李瑞生
李晓娟
柴燕涛
姜棋予
王志杰
杨锐创
冯帆
侯俊
SUN Hui-Wei;XIE Hui;LI Rui-Sheng;LI Xiao-Juan;CHAI Yan-Tao;JIANG QI-Yu;WANG Zhi-Jie;YANG Rui-Chuang;FENG Fan;HOU Jun(Research Center for Clinical and Translational Medicine;Department of Interventional Medicine Hospital of Chinese PLA, Beijing 100039, China)
出处
《生物技术通讯》
CAS
2018年第3期404-408,429,共6页
Letters in Biotechnology
关键词
进展期肝细胞癌
介入治疗
肝脏原位肿瘤模型
病理分析
advanced hepatocellular carcinoma（HCC）
interventional therapy
intrahepatic growth of HCC
pathological analysis
